Elicitation of secondary metabolites in in vitro cultures of Ammi majus L.

被引：56

作者：

Staniszewska, I

Królicka, A

Malinski, E

Lojkowska, E

Szafranek, J

机构：

[1] Univ Gdansk, Fac Biotechnol, PL-80822 Gdansk, Poland

[2] Univ Gdansk, Fac Chem, Gdansk, Poland

[3] Med Univ Gdansk, PL-80822 Gdansk, Poland

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 2003年 / 33卷 / 05期

关键词：

BION (R); dehydrogeijerin; Enterobacter sakazaki; scopoletin;

D O I：

10.1016/S0141-0229(03)00180-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The present study was concentrated on the production of secondary metabolites in callus, cell suspension and hairy roots of Anum majus L. by exposing them to elicitors: benzo(1,2,3)-thiadiazole-7-carbothionic acid S-methyl ester (BION(R)) and autoclaved lysate of cell suspension of bacteria-Enterobacter sakazaki. GC and GC-MS analysis of chloroform and methanol extracts indicated a higher accumulation of umbelliferone in the elicited tissues than in the control ones. Using GC-MS, two compounds not earlier found in A. majus tissues were identified in callus cultures: scopoletin (7-hydroxy-6-metoxy-2H-1-benzopyron-2-one) and dehydrogeijerin (7-methoxy-6-(3-methyl-1-oxo-2-butenyl)-2H-1-benzopyran-2-one). (C) 2003 Elsevier Inc. All rights reserved.

引用

页码：565 / 568

页数：4

共 29 条

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