Cloning and characterization of dnaE, encoding the catalytic subunit of replicative DNA polymerase III, from Vibrio cholerae strain C6706

被引:4
作者
Franco, AA
Yeh, PE
Johnson, JA
Barry, EM
Guerra, H
Maurer, R
Morris, JG
机构
[1] UNIV MARYLAND,SCH MED,DIV INFECT DIS,BALTIMORE,MD 21201
[2] CASE WESTERN RESERVE UNIV,DEPT MOL BIOL & MICROBIOL,CLEVELAND,OH 44106
[3] UNIV MARYLAND,SCH MED,DEPT VET AFFAIRS MED CTR,BALTIMORE,MD 21201
[4] UNIV MARYLAND,SCH MED,DEPT PATHOL,BALTIMORE,MD 21201
[5] UNIV MARYLAND,SCH MED,CTR VACCINE DEV,BALTIMORE,MD 21201
[6] UNIV PERUANA CAYETANO HEREDIA,INST TROP MED,LIMA,PERU
[7] INST NACL SALUD,LIMA,PERU
关键词
recombinant DNA; cholera; Escherichia coli; Salmonella typhimurium;
D O I
10.1016/0378-1119(96)00155-2
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
We report that Vibrio cholerae (Vc) contains a gene homologous to Escherichia coli dnaE, the structural gene for the alpha (catalytic) subunit of replicative DNA polymerase III (PoIII). Despite 24% amino acid (aa) differences in the encoded proteins, the Vc gene strongly complements an E. coli dnaE temperature sensitive (ts) mutant, indicating that all functional features essential for replication are conserved.
引用
收藏
页码:281 / 283
页数:3
相关论文
共 13 条
[1]   AN ATTEMPT TO UNIFY THE STRUCTURE OF POLYMERASES [J].
DELARUE, M ;
POCH, O ;
TORDO, N ;
MORAS, D ;
ARGOS, P .
PROTEIN ENGINEERING, 1990, 3 (06) :461-467
[2]   A COMPREHENSIVE SET OF SEQUENCE-ANALYSIS PROGRAMS FOR THE VAX [J].
DEVEREUX, J ;
HAEBERLI, P ;
SMITHIES, O .
NUCLEIC ACIDS RESEARCH, 1984, 12 (01) :387-395
[3]  
FRANCO AA, 1995, THESIS U PERUANA CAY
[4]   ISOLATION AND CHARACTERIZATION OF A 2ND RNASE-H (RNASE-HII) OF ESCHERICHIA-COLI K-12 ENCODED BY THE RNHB GENE [J].
ITAYA, M .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (21) :8587-8591
[5]   DNA-POLYMERASE-III HOLOENZYME - STRUCTURE AND FUNCTION OF A CHROMOSOMAL REPLICATING MACHINE [J].
KELMAN, Z ;
ODONNELL, M .
ANNUAL REVIEW OF BIOCHEMISTRY, 1995, 64 :171-200
[6]   NUCLEOTIDE-SEQUENCES OF DNAE, THE GENE FOR THE POLYMERASE SUBUNIT OF DNA POLYMERASE-III IN SALMONELLA-TYPHIMURIUM, AND A VARIANT THAT FACILITATES GROWTH IN THE ABSENCE OF ANOTHER POLYMERASE SUBUNIT [J].
LANCY, ED ;
LIFSICS, MR ;
MUNSON, P ;
MAURER, R .
JOURNAL OF BACTERIOLOGY, 1989, 171 (10) :5581-5586
[7]  
LI SJ, 1992, J BIOL CHEM, V267, P16841
[8]  
MAKI H, 1985, J BIOL CHEM, V260, P2987
[9]   HOLE, THE GENE CODING FOR THE THETA SUBUNIT OF DNA-POLYMERASE-III OF ESCHERICHIA-COLI - CHARACTERIZATION OF HOLE MUTANT AND COMPARISON WITH A DNAQ (EPSILON-SUBUNIT) MUTANT [J].
SLATER, SC ;
LIFSICS, MR ;
ODONNELL, M ;
MAURER, R .
JOURNAL OF BACTERIOLOGY, 1994, 176 (03) :815-821
[10]   HIGH-COPY-NUMBER AND LOW-COPY-NUMBER PLASMID VECTORS FOR LACZ-ALPHA-COMPLEMENTATION AND CHLORAMPHENICOL-RESISTANCE OR KANAMYCIN-RESISTANCE SELECTION [J].
TAKESHITA, S ;
SATO, M ;
TOBA, M ;
MASAHASHI, W ;
HASHIMOTOGOTOH, T .
GENE, 1987, 61 (01) :63-74