Cloning and characterization of dnaE, encoding the catalytic subunit of replicative DNA polymerase III, from Vibrio cholerae strain C6706

被引：4

作者：

Franco, AA

Yeh, PE

Johnson, JA

Barry, EM

Guerra, H

Maurer, R

Morris, JG

机构：

[1] UNIV MARYLAND,SCH MED,DIV INFECT DIS,BALTIMORE,MD 21201

[2] CASE WESTERN RESERVE UNIV,DEPT MOL BIOL & MICROBIOL,CLEVELAND,OH 44106

[3] UNIV MARYLAND,SCH MED,DEPT VET AFFAIRS MED CTR,BALTIMORE,MD 21201

[4] UNIV MARYLAND,SCH MED,DEPT PATHOL,BALTIMORE,MD 21201

[5] UNIV MARYLAND,SCH MED,CTR VACCINE DEV,BALTIMORE,MD 21201

[6] UNIV PERUANA CAYETANO HEREDIA,INST TROP MED,LIMA,PERU

[7] INST NACL SALUD,LIMA,PERU

来源：

GENE | 1996年 / 175卷 / 1-2期

关键词：

recombinant DNA; cholera; Escherichia coli; Salmonella typhimurium;

D O I：

10.1016/0378-1119(96)00155-2

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We report that Vibrio cholerae (Vc) contains a gene homologous to Escherichia coli dnaE, the structural gene for the alpha (catalytic) subunit of replicative DNA polymerase III (PoIII). Despite 24% amino acid (aa) differences in the encoded proteins, the Vc gene strongly complements an E. coli dnaE temperature sensitive (ts) mutant, indicating that all functional features essential for replication are conserved.

引用

页码：281 / 283

页数：3

共 13 条

[1] AN ATTEMPT TO UNIFY THE STRUCTURE OF POLYMERASES [J].