Head group specificity of phospholipase D isoenzymes from poppy seedlings (Papaver somniferum L.)

被引：28

作者：

Oblozinsky, M

Ulbrich-Hofmann, R ^{[1
]}

Bezakova, L

机构：

[1] Univ Halle Wittenberg, Dept Biochem Biotechnol, D-06120 Halle Saale, Germany

[2] Comenius Univ, Fac Pharm, Dept Cell & Mol Biol Drugs, SK-83232 Bratislava, Slovakia

来源：

BIOTECHNOLOGY LETTERS | 2005年 / 27卷 / 03期

关键词：

head group specificity; Papaver somniferum L; phospholipase D; phospholipid transformation; transesterification;

D O I：

10.1007/s10529-004-7853-x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The biocatalytical potential of two new phospholipase D (PLD) isoenzymes from poppy seedlings (Papaver somniferum L.), PLD-A and PLD-B, was examined by comparing their activities in phospholipid transformation. Both enzymes showed the same ratio in rates of hydrolysis [phosphatidylcholine (PC):phosphatidylglycerol (PG):phosphatidylserine:phosphatidylinositol=1:0.5:0.3:0.1] and were inactive towards phosphatidylethanolamine (PE). PLD-A did not catalyze head group exchange whereas PLD-B showed a high transphosphatidylation potential in the conversion of PC into PG and PE. This enzyme also catalyzed the transesterification of octadecylphosphocholine into octadecylphosphoglycerol or octadecylphosphoethanolamine.

引用

页码：181 / 185

页数：5

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