Clathrin- and caveolin-1-independent endocytosis: entry of simian virus 40 into cells devoid of caveolae

被引:362
作者
Damm, EM
Pelkmans, L
Kartenbeck, J
Mezzacasa, A
Kurzckalia, T
Helenius, A [1 ]
机构
[1] Swiss Fed Inst Technol, Inst Biochem, CH-8093 Zurich, Switzerland
[2] Max Planck Inst Mol Cell Biol & Genet, D-01307 Dresden, Germany
[3] German Canc Res Ctr, D-69120 Heidelberg, Germany
关键词
D O I
10.1083/jcb.200407113
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Simian Virus 40 (SV40) has been shown to enter host cells by caveolar endocytosis followed by transport via caveosomes to the endoplasmic reticulum (ER). Using a caveolin-1 (cav-1)-deficient cell line (human hepatoma 7) and embryonic fibroblasts from a cav-1 knockout mouse, we found that in the absence of caveolae, but also in wild-type embryonic fibroblasts, the virus exploits an alternative, cav-1-independent pathway. Internalization was rapid (t(1/2)= 20 min) and cholesterol and tyrosine kinase dependent but independent of clathrin, dynamin II, and ARF6. The viruses were internalized in small, tight-fitting vesicles and transported to membrane-bounded, pH-neutral organelles similar to caveosomes but devoid of cav-1 and -2. The viruses were next transferred by microtubule-dependent vesicular transport to the ER, a step that was required for infectivity. Our results revealed the existence of a virus-activated endocytic pathway from the plasma membrane to the ER that involves neither clathrin nor caveolae and that can be activated also in the presence of cav-1.
引用
收藏
页码:477 / 488
页数:12
相关论文
共 46 条
  • [1] Bound simian virus 40 translocates to caveolin-enriched membrane domains, and its entry is inhibited by drugs that selectively disrupt caveolae
    Anderson, HA
    Chen, YZ
    Norkin, LC
    [J]. MOLECULAR BIOLOGY OF THE CELL, 1996, 7 (11) : 1825 - 1834
  • [2] The caveolae membrane system
    Anderson, RGW
    [J]. ANNUAL REVIEW OF BIOCHEMISTRY, 1998, 67 : 199 - 225
  • [3] Recycling of raft-associated prohormone sorting receptor carboxypeptidase E requires interaction with ARF6
    Arnaoutova, I
    Jackson, CL
    Al-Awar, MS
    Donaldson, JG
    Loh, YP
    [J]. MOLECULAR BIOLOGY OF THE CELL, 2003, 14 (11) : 4448 - 4457
  • [4] Localization of autocrine motility factor receptor to caveolae and clathrin-independent internalization of its ligand to smooth endoplasmic reticulum
    Benlimame, N
    Le, PU
    Nabi, IR
    [J]. MOLECULAR BIOLOGY OF THE CELL, 1998, 9 (07) : 1773 - 1786
  • [5] Benmerah A, 1999, J CELL SCI, V112, P1303
  • [6] SORTING OF GPI-ANCHORED PROTEINS TO GLYCOLIPID-ENRICHED MEMBRANE SUBDOMAINS DURING TRANSPORT TO THE APICAL CELL-SURFACE
    BROWN, DA
    ROSE, JK
    [J]. CELL, 1992, 68 (03) : 533 - 544
  • [7] Regulated portals of entry into the cell
    Conner, SD
    Schmid, SL
    [J]. NATURE, 2003, 422 (6927) : 37 - 44
  • [8] DAUTRYVARSAT A, 2000, ENDOCYTOSIS, P26
  • [9] THE FUNCTION OF DYNAMIN IN ENDOCYTOSIS
    DECAMILLI, P
    TAKEI, K
    MCPHERSON, PS
    [J]. CURRENT OPINION IN NEUROBIOLOGY, 1995, 5 (05) : 559 - 565
  • [10] Loss of caveolae, vascular dysfunction, and pulmonary defects in caveolin-1 gene-disrupted mice
    Drab, M
    Verkade, P
    Elger, M
    Kasper, M
    Lohn, M
    Lauterbach, B
    Menne, J
    Lindschau, C
    Mende, F
    Luft, FC
    Schedl, A
    Haller, H
    Kurzchalia, TV
    [J]. SCIENCE, 2001, 293 (5539) : 2449 - 2452