Development and characterisation of a collagen nano-hydroxyapatite composite scaffold for bone tissue engineering

被引：174

作者：

Cunniffe, Grainne M. ^{[1
,3
]}

Dickson, Glenn R. ^{[3
]}

Partap, Sonia ^{[1
,4
]}

Stanton, Kenneth T. ^{[2
]}

O'Brien, Fergal J. ^{[1
,4
]}

机构：

[1] Royal Coll Surgeons Ireland, Dept Anat, Dublin 2, Ireland

[2] Univ Coll Dublin, UCD Sch Elect Elect & Mech Engn, Dublin 2, Ireland

[3] Queens Univ Belfast, Ctr Canc Res & Cell Biol, Sch Med Dent & Biomed Sci, Belfast, Antrim, North Ireland

[4] Trin Coll, Ctr Bioengn, Dublin, Ireland

来源：

JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE | 2010年 / 21卷 / 08期

基金：

爱尔兰科学基金会;

关键词：

GAG SCAFFOLDS; SIZE;

D O I：

10.1007/s10856-009-3964-1

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Bone regeneration requires scaffolds that possess suitable mechanical and biological properties. This study sought to develop a novel collagen-nHA biocomposite scaffold via two new methods. Firstly a stable nHA suspension was produced and added to a collagen slurry (suspension method), and secondly, porous collagen scaffolds were immersed in nHA suspension after freeze-drying (immersion method). Significantly stronger constructs were produced using both methods compared to collagen only scaffolds, with a high porosity maintained (> 98.9%). It was found that Coll-nHA composite scaffolds produced by the suspension method were up to 18 times stiffer than the collagen control (5.50 +/- A 1.70 kPa vs. 0.30 +/- A 0.09 kPa). The suspension method was also more reproducible, and the quantity of nHA incorporated could be varied with greater ease than with the immersion technique. In addition, Coll-nHA composites display excellent biological activity, demonstrating their potential as bone graft substitutes in orthopaedic regenerative medicine.

引用

页码：2293 / 2298

页数：6