High virus loads in naturally and experimentally SIVagm-infected African green monkeys

被引:39
作者
Holzammer, S [1 ]
Holznagel, E [1 ]
Kaul, A [1 ]
Kurth, R [1 ]
Norley, S [1 ]
机构
[1] Paul Ehrlich Inst, D-63225 Langen, Germany
关键词
AIDS; SIVagm; HIV; natural host; pathogenesis; virus load;
D O I
10.1006/viro.2001.0870
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A quantitative RT-PCR assay was developed for SIVagm and was used to measure the levels of viral RNA in the plasma of experimentally and naturally infected African green monkeys. The number of productively infected PBMCs and the number of cells carrying integrated provirus were also measured. Plasma virus loads in experimentally infected animals peaked at e weeks postinfection, ranging from 2.9 x 10(5) to 4.2 X 10(7) RNA copies/ml plasma. Set points of 2.1 x 10(3) to 2.8 x 10(6) RNA copies/ml plasma were maintained for one year. Similarly, the number of cells carrying integrated SIVagm provirus remained relatively stable in individual animals for one year with set points ranging from 73 to 810 proviral copies per 10(8) PBMC. However, the number of productively infected cells fluctuated considerably during this period. Virus loads in the 26 naturally infected AGMs ranged from 8.3 x 10(3) to 1.1 x 10(7) (mean 1.7 x 10(8)) RNA copies/ml plasma. These levels of viremia are similar to those seen in pathogenic systems (HIV-1, SIVmac), indicating that control of SIVagm replication is not the reason for the natural host's resistance to disease progression. (C) 2001 Academic Press.
引用
收藏
页码:324 / 331
页数:8
相关论文
共 29 条
[1]   SPECIES-SPECIFIC DIVERSITY AMONG SIMIAN IMMUNODEFICIENCY VIRUSES FROM AFRICAN-GREEN MONKEYS [J].
ALLAN, JS ;
SHORT, M ;
TAYLOR, ME ;
SU, S ;
HIRSCH, VM ;
JOHNSON, PR ;
SHAW, GM ;
HAHN, BH .
JOURNAL OF VIROLOGY, 1991, 65 (06) :2816-2828
[2]   MOLECULAR PROFILE OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 INFECTION IN SYMPTOMLESS PATIENTS AND IN PATIENTS WITH AIDS [J].
BAGNARELLI, P ;
MENZO, S ;
VALENZA, A ;
MANZIN, A ;
GIACCA, M ;
ANCARANI, F ;
SCALISE, G ;
VARALDO, PE ;
CLEMENTI, M .
JOURNAL OF VIROLOGY, 1992, 66 (12) :7328-7335
[3]   Simian immunodeficiency virus of African green monkeys is apathogenic in the newborn natural host [J].
Beer, B ;
Denner, J ;
Brown, CR ;
Norley, S ;
zur Megede, J ;
Coulibaly, C ;
Plesker, R ;
Holzammer, S ;
Baier, M ;
Hirsch, VM ;
Kurth, R .
JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES AND HUMAN RETROVIROLOGY, 1998, 18 (03) :210-220
[4]   Lack of dichotomy between virus load of peripheral blood and lymph nodes during long-term simian immunodeficiency virus infection of African green monkeys [J].
Beer, B ;
Scherer, J ;
Megede, JZ ;
Norley, S ;
Baier, M ;
Kurth, R .
VIROLOGY, 1996, 219 (02) :367-375
[5]   High levels of viral replication during primary simian immunodeficiency virus SIVagm infection are rapidly and strongly controlled in African green monkeys [J].
Diop, OM ;
Gueye, P ;
Dias-Tavares, M ;
Kornfeld, C ;
Faye, A ;
Ave, P ;
Huerre, M ;
Corbet, S ;
Barre-Sinoussi, F ;
Müller-Trutwin, MC .
JOURNAL OF VIROLOGY, 2000, 74 (16) :7538-7547
[6]  
ENNEN J, 1993, INT C AIDS, V9, P6
[7]   Origin of HIV-1 in the chimpanzee Pan troglodytes troglodytes [J].
Gao, F ;
Bailes, E ;
Robertson, DL ;
Chen, YL ;
Rodenburg, CM ;
Michael, SF ;
Cummins, LB ;
Arthur, LO ;
Peeters, M ;
Shaw, GM ;
Sharp, PM ;
Hahn, BH .
NATURE, 1999, 397 (6718) :436-441
[8]   HUMAN INFECTION BY GENETICALLY DIVERSE SIVSM-RELATED HIV-2 IN WEST AFRICA [J].
GAO, F ;
YUE, L ;
WHITE, AT ;
PAPPAS, PG ;
BARCHUE, J ;
HANSON, AP ;
GREENE, BM ;
SHARP, PM ;
SHAW, GM ;
HAHN, BH .
NATURE, 1992, 358 (6386) :495-499
[9]   QUANTITATION OF A LENTIVIRUS IN ITS NATURAL HOST - SIMIAN IMMUNODEFICIENCY VIRUS IN AFRICAN-GREEN MONKEYS [J].
HARTUNG, S ;
BOLLER, K ;
CICHUTEK, K ;
NORLEY, SG ;
KURTH, R .
JOURNAL OF VIROLOGY, 1992, 66 (04) :2143-2149
[10]   INDUCTION OF AIDS BY SIMIAN IMMUNODEFICIENCY VIRUS FROM AN AFRICAN-GREEN MONKEY - SPECIES-SPECIFIC VARIATION IN PATHOGENICITY CORRELATES WITH THE EXTENT OF IN-VIVO REPLICATION [J].
HIRSCH, VM ;
DAPOLITO, G ;
JOHNSON, PR ;
ELKINS, WR ;
LONDON, WT ;
MONTALI, RJ ;
GOLDSTEIN, S ;
BROWN, C .
JOURNAL OF VIROLOGY, 1995, 69 (02) :955-967