Negative-ion fast atom bombardment and electrospray ionization tandem mass spectrometry for characterization of sulfated and sialyl Lewis-type glycosphingolipids

被引:14
作者
Ii, T
Ohashi, Y
Ogawa, T
Nagai, Y
机构
[1] INST PHYS & CHEM RES,FRONTIER RES PROGRAM,WAKO,SAITAMA 35101,JAPAN
[2] SODA AROMAT CO LTD,RES & DEV LABS,NODA,CHIBA 27002,JAPAN
[3] INST PHYS & CHEM RES,LAB SYNTHET CELLULAR CHEM,WAKO,SAITAMA 35101,JAPAN
[4] UNIV TOKYO,FAC AGR,BUNKYO KU,TOKYO 113,JAPAN
关键词
sulfated Lewis(a); sulfated Lewis(x); sialyl Lewis(a); sialyl Lewis(x); glycosphingolipid; ESIMS; FABMS; CID-MS/MS;
D O I
10.1007/BF00731502
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Structural characterization of sulfated and sialyl Lewis (Le)-type glycosphingolipids performed by fast atom bombardment (FAB) and electrospray ionization (ESI) mass spectrometry is described. Both FAB and ESI collision-induced dissociation tandem mass spectrometry (CID-MS/MS) of acidic glycosphingolipids allowed identification of the sulfated or sialyl sugar, and provided information on the saccharide chain sequence. The negative-ion tandem FABMS of sulfated Le-type glycosphingolipids having the non-reducing end trisaccharide ion as the precursor can be used to differentiate the Le(a)- and Le(x)-type oligosaccharides. The ESI CID-MS/MS of multiple-charged ions provided even more detailed structural information, and some of the useful daughter ions appeared with higher m/z values than the precusor because of a lower charge-state. These methodologies can be applied to the structural analyses of glycoconjugates with much larger molecular masses and higher polarity, such as the poly-sulfated and sialyl analogues.
引用
收藏
页码:273 / 283
页数:11
相关论文
共 18 条
  • [1] COMPARISON OF L-SELECTIN AND E-SELECTIN LIGAND SPECIFICITIES - THE L-SELECTIN CAN BIND THE E-SELECTIN LIGANDS SIALYL LEX AND SIALYL LEA
    BERG, EL
    MAGNANI, J
    WARNOCK, RA
    ROBINSON, MK
    BUTCHER, EC
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1992, 184 (02) : 1048 - 1055
  • [2] RETRACTED: OLIGOSACCHARIDE LIGANDS FOR NKR-P1 PROTEIN ACTIVATE NK CELLS AND CYTOTOXICITY (Retracted article. See vol. 500, pg. 492, 2013)
    BEZOUSKA, K
    YUEN, CT
    OBRIEN, J
    CHILDS, RA
    CHAI, WG
    LAWSON, AM
    DRBAL, K
    FISEROVA, A
    POSPISIL, M
    FEIZI, T
    [J]. NATURE, 1994, 372 (6502) : 150 - 157
  • [3] A SYSTEMATIC NOMENCLATURE FOR CARBOHYDRATE FRAGMENTATIONS IN FAB-MS MS SPECTRA OF GLYCOCONJUGATES
    DOMON, B
    COSTELLO, CE
    [J]. GLYCOCONJUGATE JOURNAL, 1988, 5 (04) : 397 - 409
  • [4] TOTAL SYNTHESIS OF SULFATED LE(A) PENTAOSYL CERAMIDE
    ENDO, A
    IIDA, M
    FUJITA, S
    NUMATA, M
    SUGIMOTO, M
    NUNOMURA, S
    [J]. CARBOHYDRATE RESEARCH, 1995, 270 (02) : C9 - C13
  • [5] STRUCTURE OF UNDERIVATIZED BRANCHED OLIGOSACCHARIDES BY NEGATIVE-ION FAST-ATOM-BOMBARDMENT MASS-SPECTROMETRY
    GAROZZO, D
    IMPALLOMENI, G
    MONTAUDO, G
    SPINA, E
    [J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1992, 6 (09) : 550 - 552
  • [6] II T, 1995, J BIOCHEM-TOKYO, V118, P526
  • [7] STRUCTURAL ELUCIDATION OF UNDERIVATIZED GANGLIOSIDES BY ELECTROSPRAY-IONIZATION TANDEM MASS-SPECTROMETRY (ESIMS/MS)
    II, T
    OHASHI, Y
    NAGAI, Y
    [J]. CARBOHYDRATE RESEARCH, 1995, 273 (01) : 27 - 40
  • [8] II T, 1993, ORG MASS SPECTROM, V28, P927
  • [9] FAB CID-MS/MS CHARACTERIZATION OF TETRASACCHARIDE TRISULFATE AND TETRASULFATE DERIVED FROM THE ANTIGENIC DETERMINANT RECOGNIZED BY THE ANTI-CHONDROITIN SULFATE MONOCLONAL-ANTIBODY MO-225
    II, T
    KUBOTA, M
    HIRANO, T
    OHASHI, M
    YOSHIDA, K
    SUZUKI, S
    [J]. GLYCOCONJUGATE JOURNAL, 1995, 12 (03) : 282 - 289
  • [10] A TOTAL SYNTHESIS OF GLYCONONAOSYL CERAMIDE WITH A SIALYL DIMERIC LE(X) SEQUENCE
    IIDA, M
    ENDO, A
    FUJITA, S
    NUMATA, M
    MATSUZAKI, Y
    SUGIMOTO, M
    NUNOMURA, S
    OGAWA, T
    [J]. CARBOHYDRATE RESEARCH, 1995, 270 (02) : C15 - C19