The GUS gene of E. coli, encoding beta-glucuronidase, has been widely used as a reporter gene in plant transformation. However, beta-glucuronidase activity in transgenic wheat leaf or root tissue is rarely observed or reported. To address this question, we investigated three wheat lines transformed with the GUS reporter gene. We found all three lines expressed GUS mRNA as well as beta-glucuronidase protein in their leaf and root tissues as detected by RNA gel blot, ELISA, and immunoblot analyses. However, beta-glucuronidase enzyme activity was only detected in pollen grains from the transgenic plants. Fluorometric and histochemical assays performed in the presence of wheat tissue extracts indicated that wheat leaf and root tissues contain inhibitor(s) of beta-glucuronidase activity, but pollen grains contain much lower concentrations. Further characterizations indicated that the inhibitor( s) is of low molecular weight (< 10 kDa) and is non-proteinaceous.
机构:
Department of Agricultural Sciences, Plant Breeding and Crop Science, Royal Veterinary/Agricultural Univ., 871 Frederiksberg CDepartment of Agricultural Sciences, Plant Breeding and Crop Science, Royal Veterinary/Agricultural Univ., 871 Frederiksberg C
Foiling L.
Olesen A.
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机构:
Department of Agricultural Sciences, Plant Breeding and Crop Science, Royal Veterinary/Agricultural Univ., 871 Frederiksberg CDepartment of Agricultural Sciences, Plant Breeding and Crop Science, Royal Veterinary/Agricultural Univ., 871 Frederiksberg C
机构:
Department of Agricultural Sciences, Plant Breeding and Crop Science, Royal Veterinary/Agricultural Univ., 871 Frederiksberg CDepartment of Agricultural Sciences, Plant Breeding and Crop Science, Royal Veterinary/Agricultural Univ., 871 Frederiksberg C
Foiling L.
Olesen A.
论文数: 0引用数: 0
h-index: 0
机构:
Department of Agricultural Sciences, Plant Breeding and Crop Science, Royal Veterinary/Agricultural Univ., 871 Frederiksberg CDepartment of Agricultural Sciences, Plant Breeding and Crop Science, Royal Veterinary/Agricultural Univ., 871 Frederiksberg C