Evidence for non-proteinaceous inhibitor(s) of β-glucuronidase in wheat (Triticum aestivum L.) leaf and root tissues

被引:12
作者
Bahieldin, A [1 ]
Eissa, HF
Mahfouz, HT
Dyer, WE
Madkour, MA
Qu, RD
机构
[1] AGERI, Giza, Egypt
[2] Ain Shams Univ, Fac Agr, Dept Genet, Cairo, Egypt
[3] Montana State Univ, Dept Plant Sci & Plant Pathol, Bozeman, MT 59717 USA
[4] N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA
基金
澳大利亚研究理事会;
关键词
ss-glucuronidase inhibitor; genetic transformation; GUS; transgene; wheat;
D O I
10.1007/s11240-004-5890-8
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The GUS gene of E. coli, encoding beta-glucuronidase, has been widely used as a reporter gene in plant transformation. However, beta-glucuronidase activity in transgenic wheat leaf or root tissue is rarely observed or reported. To address this question, we investigated three wheat lines transformed with the GUS reporter gene. We found all three lines expressed GUS mRNA as well as beta-glucuronidase protein in their leaf and root tissues as detected by RNA gel blot, ELISA, and immunoblot analyses. However, beta-glucuronidase enzyme activity was only detected in pollen grains from the transgenic plants. Fluorometric and histochemical assays performed in the presence of wheat tissue extracts indicated that wheat leaf and root tissues contain inhibitor(s) of beta-glucuronidase activity, but pollen grains contain much lower concentrations. Further characterizations indicated that the inhibitor( s) is of low molecular weight (< 10 kDa) and is non-proteinaceous.
引用
收藏
页码:11 / 17
页数:7
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