Long-term effect of dietary α-linolenic acid or decosahexaenoic acid on incorporation of decosahexaenoic acid in membranes and its influence on rat heart in vivo

被引:25
作者
Ayalew-Pervanchon, Adey
Rousseau, Delphine
Moreau, Daniel
Assayag, Patrick
Weill, Pierre
Grynberg, Alain
机构
[1] Univ Paris Sud 11, INRA, Univ Paris Sud, LMRFCV,Fac Pharm,UMR 1154, F-92290 Chatenay Malabry, France
[2] Univ Paris Sud, Fac Pharm, Inst Fed Rech 141, Chatenay Malabry, France
[3] Fac Med, Lab Pathophysiol & Pharmacol Cardiovasc Expt, Dijon, France
[4] Assistance Publ Hop Paris, Le Kremlin Bicetre, France
[5] Soc VALOREX, Combourtille, France
来源
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY | 2007年 / 293卷 / 04期
关键词
cardiac docosahexaenoic acid; heart function;
D O I
10.1152/ajpheart.00194.2007
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The present study was designed to evaluate whether long-term intake of dietary alpha-linolenic acid (ALA), supplied as whole grain-extruded linseed, can increase endogenous production of n-3 long-chain polyunsaturated fatty acids (FAs) in healthy adult rats and influence the heart rate (HR) and adrenergic response in the same way as docosahexaenoic acid (DHA)-rich diets. DHA enrichment was evaluated using FA analysis of tissue phospholipids after 8, 16, 24, and 32 wk of feeding in male Wistar rats randomly assigned to three dietary groups (n = 8 in each group): a reference fat diet (RFD), an ALA- rich (ALA) diet, and a DHA- rich (DHA) diet. At 1 wk before the animals were killed, under anesthesia, HR was measured from ECG recordings during an adrenergic stimulation challenge (n = 8). There was a significant increase of DHA in the cardiac membrane in the ALA group compared with the RFD group. DHA content in the cardiac membrane was similar to 10% in the ALA group vs. 20% in the DHA group and 4% in the RFD group. The cardiac FA profile was established after 2 mo and remained essentially unchanged thereafter. Regardless of the diet, DHA in the heart decreased with age. Nevertheless, DHA content in the heart remained at > 15% in the DHA group and remained greater in older rats fed the ALA diet than in younger RFD- fed rats. Basal HR decreased in the ALA group (395 +/- 24.9 beats/min) to a level between that of the DHA and RFD groups (375 +/- 26.4 and 407 +/- 36.7 beats/min, respectively). Both n-3 dietary intakes contribute to enhancement of the chronotropic response to adrenergic agonist stimulation. Regulation of HR by neurohumoral mediators may be controlled by lower content of DHA, e. g., by a dietary supply of extruded linseed (ALA).
引用
收藏
页码:H2296 / H2304
页数:9
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