GreA and GreB proteins revive backtracked RNA polymerase in vivo by promoting transcript trimming

被引:78
作者
Toulmé, F
Mosrin-Hauman, C
Sparkowski, J
Das, A
Leng, M
Rahmouni, AR
机构
[1] CNRS, Ctr Biophys Mol, F-45071 Orleans 2, France
[2] Univ Connecticut, Ctr Hlth, Dept Microbiol, Farmington, CT 06030 USA
关键词
elongation factors; GreA; GreB; RNA polymerase; transcription elongation complex;
D O I
10.1093/emboj/19.24.6853
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The GreA and GreB proteins of Escherichia coli show a multitude of effects on transcription elongation in vitro, yet their physiological functions are poorly understood. Here, we investigated whether and how these factors influence lateral oscillations of RNA polymerase (RNAP) in vivo, observed at a protein readblock, When RNAP is stalled within an (ATC/TAG), sequence, it appears to oscillate between an upstream and a downstream position on the template, 3 bp apart, with concomitant trimming of the transcript 3' terminus and its re-synthesis. Using a set of mutant E.coli strains, we show that the presence of GreA or GreB in the cell is essential to induce this trimming. We show further that in contrast to a ternary complex that is stabilized at the downstream position, the oscillating complex relies heavily on the GreA/GreB-induced 'cleavage-and-restart' process to become catalytically competent. Clearly, by promoting transcript shortening and re-alignment of the catalytic register, the Gre factors function in vivo to rescue RNAP from being arrested at template positions where the lateral stability of the ternary complex is impaired.
引用
收藏
页码:6853 / 6859
页数:7
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