Transcriptional analysis of the 69-kb sequence centromeric to HLA-J: A dense and complex structure of five genes

被引：9

作者：

Coriton, O ^{[1
]}

Lepourcelet, M ^{[1
]}

Hampe, A ^{[1
]}

Galibert, F ^{[1
]}

Mosser, J ^{[1
]}

机构：

[1] Fac Med, CNRS Recombinaisons Genet, UPR 41, F-35043 Rennes, France

来源：

MAMMALIAN GENOME | 2000年 / 11卷 / 12期

关键词：

D O I：

10.1007/s003350010213

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Performed within the framework of the sequencing of the 356-kb MHC class I distal region, systematic bioinformatic annotation and preliminary experiments conducted on the whole sequence indicate a high level and a complex pattern of expression. In this paper, we analyze a particular stretch of 69 kb centromeric to the HLA-J gene, in which we identify 21 different mRNAs mainly expressed in testis, and characterize five different transcription units, HZFw, HZFc, HCGV, HTEX6, and HTEX4. These tightly linked genes form a cluster conserved between human and mouse and displaying a high gene density of about one every 14 kb. Alternative splicing processes are observed for all the genes, together with an alternative polyadenylation event for gene HTEX4, sense/antisense mRNA overlaps for HZFw and HZFc, for HZFw and HCGV at their 3' end, and for HTEX6 and HTEX4 at their 5' end. This complex genomic structure suggests a mechanism of coregulation by cia-interaction in gene expression.

引用

页码：1127 / 1131

页数：5

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