Probing the binding sites of exchanged chlorophyll a in LN2 by Raman and site-selection fluorescence spectroscopies

被引：18

作者：

Gall, A ^{[1
]}

Robert, B

Cogdell, RJ

Bellissent-Funel, MC

Fraser, NJ

机构：

[1] CEA Saclay, Leon Brillouin Lab, CNRS, F-91191 Gif Sur Yvette, France

[2] CEA Saclay, Sect Biophys Prot & Membranes, DBCM, F-91191 Gif Sur Yvette, France

[3] CEA Saclay, URA2096, CNRS, F-91191 Gif Sur Yvette, France

[4] Univ Glasgow, IBLS, Div Biochem & Mol Biol, Glasgow G12 8QQ, Lanark, Scotland

来源：

FEBS LETTERS | 2001年 / 491卷 / 1-2期

基金：

英国生物技术与生命科学研究理事会;

关键词：

bacteriochlorophyll; chlorophyll; fluorescence line-narrowing spectroscopy; Raman spectroscopy; photosynthesis;

D O I：

10.1016/S0014-5793(01)02161-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this work we have selectively released the 800 nm absorbing bacteriochlorophyll a molecules of the LH2 protein from the photosynthetic bacterium Rhodopseudomonas acidophila, strain 10050, and replaced them with chlorophyll a (Chla), A combination of low-temperature electronic absorption, resonance Raman and site-selection fluorescence spectroscopies revealed that. the Chile pigments are indeed bound in the B800 binding site; this is the first work that formally proves that such non-native chlorine can be inserted correctly into LH2. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：143 / 147

页数：5

共 27 条

[1] Estimation of pigment stoichiometries in photosynthetic systems of purple bacteria: Special reference to the (absence of) second carotenoid in LH2 [J].

Arellano, JB ;

Raju, BB ;

Naqvi, KR ;

Gillbro, T .

PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1998, 68 (01) :84-87

[2]

Cogdell RJ, 1998, PROG BIOPHYS MOL BIO, V70, pI

[3] The structure and function of the LH2 (B800-850) complex from the purple photosynthetic bacterium Rhodopseudomonas acidophila strain 10050 [J].

Cogdell, RJ ;

Isaacs, NW ;

Freer, AA ;

Arellano, JB ;

Howard, TD ;

Papiz, MZ ;

Hawthornthwaite-Lawless, AM ;

Prince, S .

PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY, 1997, 68 (01) :1-27

[4] PROBING THE B800 BACTERIOCHLOROPHYLL BINDING-SITE OF THE ACCESSORY LIGHT-HARVESTING COMPLEX FROM RHODOBACTER-SPHAEROIDES USING SITE-DIRECTED MUTANTS .1. MUTAGENESIS, EFFECTS ON BINDING, FUNCTION AND ELECTROCHROMIC BEHAVIOR OF ITS CAROTENOIDS [J].

CRIELAARD, W ;

VISSCHERS, RW ;

FOWLER, GJS ;

VANGRONDELLE, R ;

HELLINGWERF, KJ ;

HUNTER, CN .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 1994, 1183 (03) :473-482

[5] GENETICALLY MODIFIED PHOTOSYNTHETIC ANTENNA COMPLEXES WITH BLUESHIFTED ABSORBENCY BANDS [J].

FOWLER, GJS ;

VISSCHERS, RW ;

GRIEF, GG ;

VANGRONDELLE, R ;

HUNTER, CN .

NATURE, 1992, 355 (6363) :848-850

[6] BLUE SHIFTS IN BACTERIOCHLOROPHYLL ABSORBENCY CORRELATE WITH CHANGED HYDROGEN-BONDING PATTERNS IN LIGHT-HARVESTING 2 MUTANTS OF RHODOBACTER-SPHAEROIDES WITH ALTERATIONS AT ALPHA-TYR-44 AND ALPHA-TYR-45 [J].

FOWLER, GJS ;

SOCKALINGUM, GD ;

ROBERT, B ;

HUNTER, CN .

BIOCHEMICAL JOURNAL, 1994, 299 :695-700

[7] Selective release, removal, and reconstitution of bacteriochlorophyll a molecules into the B800 sites of LH2 complexes from Rhodopseudomonas acidophila 10050 [J].

Fraser, NJ ;

Dominy, PJ ;

Ücker, B ;

Simonin, I ;

Scheer, H ;

Cogdell, RJ .

BIOCHEMISTRY, 1999, 38 (30) :9684-9692

[8]

FRASER NJ, 1998, THESIS U GLASGOW GLA

[9] Bacteriochlorin-protein interactions in native B800-B850, B800 deficient and B800-Bchlap-reconstituted complexes from Rhodopseudomonas acidophila, strain 10050 [J].

Gall, A ;

Fraser, NJ ;

Bellissent-Funel, MC ;

Scheer, H ;

Robert, B ;

Cogdell, RJ .

FEBS LETTERS, 1999, 449 (2-3) :269-272

[10] Influence of the protein binding site on the absorption properties of the monomeric bacteriochlorophyll in Rhodobacter sphaeroides LH2 complex [J].

Gall, A ;

Fowler, GJS ;

Hunter, CN ;

Robert, B .

BIOCHEMISTRY, 1997, 36 (51) :16282-16287

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