Molecular cloning of two novel types of peptidylarginine deiminase cDNAs from retinoic acid-treated culture of a newborn rat keratinocyte cell line

被引：37

作者：

Ishigami, A

Kuramoto, M

Yamada, M

Watanabe, K

Senshu, T

机构：

[1] Tokyo Metropolitan Inst Gerontol, Dept Cell Chem, Itabashi Ku, Tokyo 1730015, Japan

[2] Yokohama City Univ, Grad Sch Integrated Sci, Kanazawa Ku, Yokohama, Kanagawa 2360027, Japan

[3] Tokyo Metropolitan Inst Gerontol, Dept Expt Biol, Itabashi Ku, Tokyo 1730015, Japan

来源：

FEBS LETTERS | 1998年 / 433卷 / 1-2期

关键词：

peptidylarginine deiminase; all-trans retinoic acid; keratinocyte differentiation;

D O I：

10.1016/S0014-5793(98)00893-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Peptidylarginine deiminases (PADs) are a group of enzymes which convert protein arginine residues to citrulline residues. Using rat muscle PAD cDNA as a probe, we obtained two novel cDNAs, PAD-R11 and PAD-R4, from immortalized rat keratinocytes treated with all-trans retinoic acid. Comparison of the deduced amino acid sequences with those of muscle and hair follicle enzymes showed high conservation in the C-terminal region, Recombinant proteins encoded by both PAD-R11 and PAD-R4 showed the enzyme activities. That of PAD-R11 showed a characteristic feature of the enzyme found in the epidermis, (C) 1998 Federation of European Biochemical Societies.

引用

页码：113 / 118

页数：6

共 24 条

[1] Selective deimination of vimentin in calcium ionophore-induced apoptosis of mouse peritoneal macrophages [J].