Molecular cloning of the murine branched chain alpha-ketoacid dehydrogenase E2 subunit: Presence of 3' B1 repeat elements

被引：7

作者：

Costeas, PA ^{[1
]}

Tonelli, LA ^{[1
]}

Chinsky, JM ^{[1
]}

机构：

[1] UNIV MARYLAND,DEPT PEDIAT,DIV HUMAN GENET,SCH MED,BALTIMORE,MD 21201

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1996年 / 1305卷 / 1-2期

关键词：

branched-chain alpha-ketoacid dehydrogenase; dihydrolipoyl transacylase; E2; subunit; B1; repeat;

D O I：

10.1016/0167-4781(95)00212-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cDNA sequence encoding the murine E2 subunit (dihydrolipoyl transacylase) of the branched-chain alpha-ketoacid dehydrogenase (BCKAD) complex was determined. In the region encoding the mature E2 subunit protein, both the nucleotide composition and predicted amino acid sequence are highly conserved between murine, human, and bovine species. In contrast, the 5' sequence encoding the amino-terminal preprotein sequence and 3' untranslated region are less well conserved. The 3'-noncoding region contains sequences highly homologous to the rodent B1 repeat elements, which are related to human Alu repeat sequences. This finding is similar to the presence of three Alu repeat sequences in the 3'-noncoding region of human E2 cDNA.

引用

页码：25 / 28

页数：4

共 16 条

[1]

CHICCO AG, 1994, J BIOL CHEM, V269, P19427

[2] NONCOORDINATED RESPONSES OF BRANCHED-CHAIN ALPHA-KETOACID DEHYDROGENASE SUBUNIT GENES TO DIETARY-PROTEIN [J].

CHINSKY, JM ;

BOHLEN, LM ;

COSTEAS, PA .

FASEB JOURNAL, 1994, 8 (01) :114-120

[3]

CHINSKY JM, 1983, BIOCHIM BIOPHYS ACTA, V1216, P499

[4]

DANNER DJ, 1989, J BIOL CHEM, V264, P7742

[5] BRANCHED-CHAIN AMINO-ACID-METABOLISM [J].

HARPER, AE ;

MILLER, RH ;

BLOCK, KP .

ANNUAL REVIEW OF NUTRITION, 1984, 4 :409-454

[6]

HARRIS R, 1989, ADV ENZYME REGUL, V30, P245

[7] A FUNDAMENTAL DIVISION IN THE ALU FAMILY OF REPEATED SEQUENCES [J].

JURKA, J ;

SMITH, T .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (13) :4775-4778

[8] CONSERVATION OF PRIMARY STRUCTURE IN THE LIPOYL-BEARING AND DIHYDROLIPOYL DEHYDROGENASE BINDING DOMAINS OF MAMMALIAN BRANCHED-CHAIN ALPHA-KETO ACID DEHYDROGENASE COMPLEX - MOLECULAR-CLONING OF HUMAN AND BOVINE TRANSACYLASE (E2) CDNA [J].

LAU, KS ;

GRIFFIN, TA ;

HU, CWC ;

CHUANG, DT .

BIOCHEMISTRY, 1988, 27 (06) :1972-1981

[9] THE COMPLETE CDNA SEQUENCE FOR DIHYDROLIPOYL TRANSACYLASE (E2) OF HUMAN BRANCHED-CHAIN ALPHA-KETO ACID DEHYDROGENASE COMPLEX [J].

LAU, KS ;

CHUANG, JL ;

HERRING, WJ ;

DANNER, DJ ;

COX, RP ;

CHUANG, DT .

BIOCHIMICA ET BIOPHYSICA ACTA, 1992, 1132 (03) :319-321

[10] SUCCESSIVE WAVES OF FIXATION OF B1 VARIANTS IN RODENT LINEAGE HISTORY [J].

QUENTIN, Y .

JOURNAL OF MOLECULAR EVOLUTION, 1989, 28 (04) :299-305

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