DNA Origami Nanoneedles on Freestanding Lipid Membranes as a Tool To Observe Isotropic-Nematic Transition in Two Dimensions

被引：38

作者：

Czogalla, Aleksander ^{[1
,2
,3
]}

Kauert, Dominik J. ^{[4
]}

Seidel, Ralf ^{[4
]}

Schwille, Petra ^{[5
]}

Petrov, Eugene P. ^{[5
]}

机构：

[1] Tech Univ Dresden, Paul Langerhans Inst, Biochim Membranes Lab, D-01307 Dresden, Germany

[2] German Ctr Diabet Res DZD, D-85764 Neuherberg, Germany

[3] Univ Wroclaw, Fac Biotechnol, Dept Cytobiochem, PL-50383 Wroclaw, Poland

[4] Univ Munster, Inst Mol Cell Biol, D-48149 Munster, Germany

[5] Max Planck Inst Biochem, Dept Cellular & Mol Biophys, D-82152 Martinsried, Germany

来源：

NANO LETTERS | 2015年 / 15卷 / 01期

基金：

欧洲研究理事会;

关键词：

DNA origami; lipid membrane; fluorescence correlation spectroscopy; translational diffusion; rotational diffusion; isotropic-nematic transition; AMYLOID FIBRILS; FOLDING DNA; SURFACE; CURVATURE; VESICLES; BEHAVIOR; ART;

D O I：

10.1021/nl504158h

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We introduce a simple experimental system to study dynamics of needle-like nanoobjects in two dimensions (2D) as a function of their surface density close to the isotropicnematic transition. Using fluorescence correlation spectroscopy, we find that translational and rotational diffusion of rigid DNA origami nanoneedles bound to freestanding lipid membranes is strongly suppressed upon an increase in the surface particle density. Our experimental observations show a good agreement with results of Monte Carlo simulations of Brownian hard needles in 2D.

引用

页码：649 / 655

页数：7

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