Contrast and depth enhancement in two-photon microscopy of human skin ex vivo by use of optical clearing agents

被引：103

作者：

Cicchi, R

Pavone, FS

Massi, D

Sampson, DD

机构：

[1] Univ Florence, LENS, I-50019 Florence, Italy

[2] Univ Florence, Dept Phys, I-50019 Florence, Italy

[3] Univ Florence, Dept Human Pathol & Oncol, I-50134 Florence, Italy

[4] Univ Western Australia, Sch Elect Elect & Comp Engn, Opt Biomed Engn Lab, Crawley, WA 6009, Australia

来源：

OPTICS EXPRESS | 2005年 / 13卷 / 07期

关键词：

D O I：

10.1364/OPEX.13.002337

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We investigate the application of hyperosmotic optical clearing agents to improve the image contrast and penetration depth in two-photon microscopy of human dermis ex vivo. We show that the agents glycerol, propylene glycol, and glucose all convey significant improvements and we provide results on their dynamic behaviour and the reversibility of the effect. At suitable concentrations, such agents have the potential to be compatible with living tissue and may possibly enhance in-vivo deep-tissue imaging. (C) 2005 Optical Society of America.

引用

页码：2337 / 2344

页数：8

共 21 条

[1]

[Anonymous], 1999, HISTOLOGICAL HISTOCH

[2] REFRACTOMETRY AND INTERFEROMETRY OF LIVING CELLS [J].