Bottom-up genome assembly using the Bacillus subtilis genome vector

被引：99

作者：

Itaya, Mitsuhiro ^{[1
,2
]}

Fujita, Kyoko ^{[1
]}

Kuroki, Azusa ^{[1
,2
]}

Tsuge, Kenji ^{[1
,2
]}

机构：

[1] Mitsubishi Kagaku Inst Life Sci, Machida, Tokyo 1958511, Japan

[2] Keio Univ, Inst Adv Biosci, Nihonko Ku, Tsuruoka Shi, Yamagata 9970017, Japan

来源：

NATURE METHODS | 2008年 / 5卷 / 01期

关键词：

D O I：

10.1038/NMETH1143

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We established a protocol to construct complete recombinant genomes from their small contiguous DNA pieces and obtained the genomes of mouse mitochondrion and rice chloroplast using a B. subtilis genome (BGM) vector. This method allows the design of any recombinant genomes, valuable not only for fundamental research in systems biology and synthetic biology but also for various applications in the life sciences.

引用

页码：41 / 43

页数：3

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