Signaling by human herpesvirus 8 kaposin A through direct membrane recruitment of cytohesin-1

被引：87

作者：

Kliche, S

Nagel, W

Kremmer, E

Atzler, C

Ege, A

Knorr, T

Koszinowski, U

Kolanus, W

Haas, J

机构：

[1] Univ Munich, Max Von Pettenkofer Inst, Genzentrum, D-81377 Munich, Germany

[2] Univ Munich, Mol Biol Lab, Genzentrum, D-81377 Munich, Germany

[3] GSF Forschungszentrum Umwelt & Gesundheit GMBH, Inst Mol Immunol, D-81377 Munich, Germany

来源：

MOLECULAR CELL | 2001年 / 7卷 / 04期

关键词：

D O I：

10.1016/S1097-2765(01)00227-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The induction of a transformed cellular phenotype by viruses requires the modulation of signaling pathways through viral proteins. We show here that the phenotypic changes induced by the kaposin A protein of human herpesvirus 8 are mediated through its direct interaction with cytohesin-1, a guanine nucleotide exchange factor for ARF GTPases and regulator of integrin-mediated cell adhesion. Focus formation, stress fiber dissolution, and activation of the ERK-1/2 MAP kinase signal cascade were reverted by the cytohesin-1 E157K mutant, which is deficient in catalyzing guanine nucleotide exchange. Furthermore, liposome-embedded kaposin A specifically stimulates cytohesin-1 dependent GTP binding of myristoylated ARF1 in vitro. These results suggest a previously unknown involvement of ARF GTPases in the control of cellular functions by herpesviruses.

引用

页码：833 / 843

页数：11