AIK-C measles vaccine expressing fusion protein of respiratory syncytial virus induces protective antibodies in cotton rats

被引:23
作者
Sawada, Akihito [1 ]
Komase, Katsuhiro [2 ]
Nakayama, Tetsuo [1 ]
机构
[1] Kitasato Univ, Kitasato Inst Life Sci, Lab Viral Infect 1, Minato Ku, Tokyo 1088641, Japan
[2] Natl Inst Infect Dis, Dept Virol 3, Tokyo 2080011, Japan
关键词
Measles virus (MV); Respiratory syncytial virus (RSV); Cotton rat; Neutralizing antibodies; IMMUNE-RESPONSES; YOUNG-CHILDREN; CONFERS PROTECTION; CLONED CDNA; INFANTS; RSV; INFECTION; STRAIN; LIVE; GLYCOPROTEIN;
D O I
10.1016/j.vaccine.2010.12.028
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Respiratory syncytial virus (RSV) is the most common cause of respiratory infection in infants, and no vaccine is available. In this report, recombinant AIK-C measles vaccines, expressing the RSV G or F protein of subgroup A (MVAIK/RSV/G or F), were investigated as a RSV vaccine candidate. MVAIK/RSV/G or F had the original ts phenotype and expressed RSV/G or F protein. Cross-reactive neutralizing antibodies against RSV subgroups A and B were detected in cotton rats immunized intramuscularly with MVAIK/RSV/F but not MVAIK/RSV/G. In cotton rats infected with RSV, RSV was recovered and lung histopathological finding was compatible with interstitial pneumonia, demonstrating thickening of alveolar walls and infiltration of mononuclear cells. When cotton rats immunized with MVAIK/RSV/F were challenged with homologous RSV subgroup A. no infectious RSV was recovered and very mild inflammation was noted without RSV antigen expression. When they were challenged with subgroup B, protective efficacy decreased. When cotton rats immunized with MVAIK/RSV/G were challenged with RSV subgroup A, low levels of infectious virus were recovered from lung. When challenged with subgroup B, no protective effects was demonstrated, demonstrating large amounts of RSV antigen in bronchial-epithelial cells. MVAIK/RSV/F is promising candidate and protective effects should be confirmed in monkey model. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1481 / 1490
页数:10
相关论文
共 46 条
[11]   Vaccination of infant macaques with a recombinant modified vaccinia virus Ankara expressing the respiratory syncytial virus F and G genes does not predispose for immunopathology [J].
de Waal, L ;
Wyatt, LS ;
Yüksel, S ;
van Amerongen, G ;
Moss, B ;
Niesters, HGM ;
Osterhaus, ADME ;
de Swart, RL .
VACCINE, 2004, 22 (08) :923-926
[12]   A vectored measles virus induces hepatitis B surface antigen antibodies while protecting Macaques against measles virus challenge [J].
del Valle, Jorge Reyes ;
Devaux, Patricia ;
Hodge, Gregory ;
Wegner, Nicholas J. ;
McChesney, Michael B. ;
Cattaneo, Roberto .
JOURNAL OF VIROLOGY, 2007, 81 (19) :10597-10605
[13]   Live measles vaccine expressing the secreted form of the West Nile virus envelope glycoprotein protects against West Nile virus encephalitis [J].
Desprès, P ;
Combredet, C ;
Frenkiel, MP ;
Lorin, C ;
Brahic, M ;
Tangy, F .
JOURNAL OF INFECTIOUS DISEASES, 2005, 191 (02) :207-214
[14]   Respiratory syncytial virus pneumonitis in immunocompromised adults: Clinical features and outcome [J].
Ebbert, JO ;
Limper, AH .
RESPIRATION, 2005, 72 (03) :263-269
[15]   Passive IgA monoclonal antibody is no more effective than IgG at protecting mice from mucosal challenge with respiratory syncytial virus [J].
Fisher, RG ;
Crowe, JE ;
Johnson, TR ;
Tang, YW ;
Graham, BS .
JOURNAL OF INFECTIOUS DISEASES, 1999, 180 (04) :1324-1327
[16]   Impairment of T Cell Immunity by the Respiratory Syncytial Virus: Targeting Virulence Mechanisms for Therapy and Prophylaxis [J].
Gonzalez, P. A. ;
Bueno, S. M. ;
Riedel, C. A. ;
Kalergis, A. M. .
CURRENT MEDICINAL CHEMISTRY, 2009, 16 (34) :4609-4625
[17]   Analysis of antibody response by temperature-sensitive measles vaccine strain in the cotton rat model [J].
Haga, Takeshi ;
Murayama, Niho ;
Shimizu, Yuya ;
Saito, Akatsuki ;
Sakamoto, Takumi ;
Morita, Tetsuo ;
Komase, Katsuhiro ;
Nakayama, Tetsuo ;
Uchida, Kazuyuki ;
Katayama, Tetsuro ;
Shinohara, Akio ;
Koshimoto, Chihiro ;
Sato, Hiroshi ;
Miyata, Hironori ;
Katahira, Kiyoaki ;
Goto, Yoshitaka .
COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES, 2009, 32 (05) :395-406
[18]   RESPIRATORY-SYNCYTIAL-VIRUS INFECTIONS, RE-INFECTIONS AND IMMUNITY - PROSPECTIVE, LONGITUDINAL-STUDY IN YOUNG-CHILDREN [J].
HENDERSON, FW ;
COLLIER, AM ;
CLYDE, WA ;
DENNY, FW .
NEW ENGLAND JOURNAL OF MEDICINE, 1979, 300 (10) :530-534
[19]   Human PIV-2 recombinant Sendai virus (rSeV) elicits durable immunity and combines with two additional rSeVs to protect against hPIV-1, hPIV-2, hPIV-3, and RSV [J].
Jones, Bart ;
Zhan, Xiaoyan ;
Mishin, Vasiliy ;
Slobod, Karen S. ;
Surman, Sherri ;
Russell, Charles J. ;
Portner, Allen ;
Hurwitz, Julia L. .
VACCINE, 2009, 27 (12) :1848-1857
[20]   AN EPIDEMIOLOGIC STUDY OF ALTERED CLINICAL REACTIVITY TO RESPIRATORY SYNCYTIAL (RS) VIRUS INFECTION IN CHILDREN PREVIOUSLY VACCINATED WITH AN INACTIVATED RS VIRUS VACCINE [J].
KAPIKIAN, AZ ;
MITCHELL, RH ;
CHANOCK, RM ;
SHVEDOFF, RA ;
STEWART, CE .
AMERICAN JOURNAL OF EPIDEMIOLOGY, 1969, 89 (04) :405-+