A method for the selection of production media for actinomycete strains based on their metabolite HPLC profiles

被引：29

作者：

Tormo, JR ^{[1
]}

García, JB ^{[1
]}

DeAntonio, M ^{[1
]}

Feliz, J ^{[1
]}

Mira, A ^{[1
]}

Díez, MT ^{[1
]}

Hernández, P ^{[1
]}

Peláez, F ^{[1
]}

机构：

[1] CIBE, Merck Res Labs, Madrid 28027, Spain

来源：

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY | 2003年 / 30卷 / 10期

关键词：

automation; HPLC; media actinomycetes; secondary metabolites; diversity;

D O I：

10.1007/s10295-003-0084-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The manipulation of growth conditions of microorganisms is a common strategy used by pharmaceutical companies to improve the quantities and spectra of secondary metabolites with potential therapeutic interest. In this work, the effects of fermentation media on secondary metabolite production from a set of Actinomycetes was statistically compared. For this purpose, we created an automated method for comparing the ability of microorganisms to produce different secondary metabolites. HPLC analyses guided the selection of those media in which a wider chemical diversity was obtained from microorganisms inoculated in a wide spectrum of production media. Fermented media yielding a better secondary metabolite profile were included in subsequent drug discovery screening.

引用

页码：582 / 588

页数：7

共 15 条

[1] Finding the needle in the haystack. The dereplication of natural product extracts [J].