Neutral and acidic oligosaccharides in Holstein-Friesian colostrum during the first 3 days of lactation measured by high performance liquid chromatography on a microfluidic chip and time-of-flight mass spectrometry

被引:93
作者
Barile, D. [1 ]
Marotta, M. [2 ]
Chu, C. [3 ,4 ]
Mehra, R. [2 ]
Grimm, R. [5 ]
Lebrilla, C. B. [3 ,4 ]
German, J. B. [1 ]
机构
[1] Univ Calif Davis, Dept Food Sci & Technol, Davis, CA 95616 USA
[2] TEAGASC, Moorepk Food Res Ctr, Fermoy, Cork, Ireland
[3] Univ Calif Davis, Dept Chem, Davis, CA 95616 USA
[4] Univ Calif Davis, Dept Biochem & Mol Med, Davis, CA 95616 USA
[5] Agilent Technol, Santa Clara, CA 95052 USA
关键词
oligosaccharide; Holstein-Friesian colostrum; high performance liquid chromatography; time-of-flight mass spectrometry; BOVINE-MILK; SIALYLOLIGOSACCHARIDES; GLYCOPROTEINS; INHIBITION; BINDING; CELLS;
D O I
10.3168/jds.2010-3156
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
Oligosaccharides (OS) from bovine milk are a class of bioactive molecules that are receiving increasing commercial attention for their potential health benefits. In the present work we measured, comprehensively and systematically, free milk OS in the colostrum of 7 Holstein-Friesian cows during the first 3 d of lactation in 12-h intervals by HPLC-chip/time-of-flight mass spectrometry to determine the biological variation of free milk OS in early lactation. The high sensitivity and resolution of the analytical technique made it possible to monitor all OS species, thus providing a comprehensive and quantitative analysis of OS variations during colostrum production. This study confirmed that although sialyllactose is the major OS in bovine colostrum, several neutral OS species are present in significant abundance even at the third day of lactation. Furthermore, variation in terms of OS species and relative abundances of OS between cows suggest individual animal variation. These variations are likely due to genetic factors because environmental factors such as nutrition, lactation number, and accommodation were the same for all cows. This investigation revealed that colostrum milk from Holstein-Friesian cows is a rich source of neutral and acidic OS for the food and pharmaceutical industries.
引用
收藏
页码:3940 / 3949
页数:10
相关论文
共 21 条
[1]   Oligosaccharides from milk [J].
Boehm, Gunther ;
Stahl, Bernd .
JOURNAL OF NUTRITION, 2007, 137 (03) :847S-849S
[2]   The first prebiotics in humans - Human milk oligosaccharides [J].
Coppa, GV ;
Bruni, S ;
Morelli, L ;
Soldi, S ;
Gabrielli, O .
JOURNAL OF CLINICAL GASTROENTEROLOGY, 2004, 38 (06) :S80-S83
[3]   Offline coupling of low-pressure anion-exchange chromatography with MALDI-MS to determine the elution order of human milk oligosaccharides [J].
Finke, B ;
Mank, M ;
Daniel, H ;
Stahl, B .
ANALYTICAL BIOCHEMISTRY, 2000, 284 (02) :256-265
[4]  
Gopal PK, 2000, BRIT J NUTR, V84, pS69
[5]   UNDIALYZABLE GROWTH-FACTORS FOR LACTOBACILLUS-BIFIDUS VAR PENNSYLVANICUS - PROTECTIVE EFFECT OF SIALIC-ACID BOUND TO GLYCOPROTEINS AND OLIGOSACCHARIDES AGAINST BACTERIAL-DEGRADATION [J].
GYORGY, P ;
JEANLOZ, RW ;
VONNICOL.H ;
ZILLIKEN, F .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1974, 43 (01) :29-33
[6]   BIFIDUS FACTOR .1. A VARIANT OF LACTOBACILLUS-BIFIDUS REQUIRING A SPECIAL GROWTH FACTOR [J].
GYORGY, P ;
NORRIS, RF ;
ROSE, CS .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1954, 48 (01) :193-201
[7]   Glycoprofiling of bifidobacterial consumption of human milk oligosaccharides demonstrates strain specific, preferential consumption of small chain glycans secreted in early human lactation [J].
Locascio, Riccardo G. ;
Ninonuevo, Milady R. ;
Freeman, Samara L. ;
Sela, David A. ;
Grimm, Rudolf ;
Lebrilla, Carlito B. ;
Mills, David A. ;
German, J. Bruce .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2007, 55 (22) :8914-8919
[8]   Sialyloligosaccharides in human and bovine milk and in infant formulas:: Variations with the progression of lactation [J].
Martín-Sosa, S ;
Martín, MJ ;
García-Pardo, LA ;
Hueso, P .
JOURNAL OF DAIRY SCIENCE, 2003, 86 (01) :52-59
[9]   Goats' milk as a natural source of lactose-derived oligosaccharides: Isolation by membrane technology [J].
Martinez-Ferez, A ;
Rudloff, S ;
Guadix, A ;
Henkel, CA ;
Pohlentz, G ;
Boza, JJ ;
Guadix, EM ;
Kunz, C .
INTERNATIONAL DAIRY JOURNAL, 2006, 16 (02) :173-181
[10]   Bovine sialyl oligosaccharides: seasonal variations in their concentrations in milk, and a comparison of the colostrums of Jersey and Friesian cows [J].
McJarrow, P ;
van Amelsfort-Schoonbeek, J .
INTERNATIONAL DAIRY JOURNAL, 2004, 14 (07) :571-579