Potent inhibition of human influenza H5N1 virus by oligonucleotides derived by SELEX

被引：71

作者：

Cheng, Congsheng ^{[1
,2
]}

Dong, Jie ^{[1
]}

Yao, Lihong

Chen, Aijun ^{[2
]}

Jia, Runqing ^{[2
]}

Huan, Lifang ^{[2
]}

Guo, Jianqlang ^{[2
]}

Shu, Yuelong ^{[1
]}

Zhang, Zhiqing ^{[2
]}

机构：

[1] Chinese Ctr Dis Control & Prevent, Natl Inst Viral Dis Control & Prevent, State Key Lab Infect Dis Prevent & Control, Beijing 100052, Peoples R China

[2] Chinese Ctr Dis Control & Prevent, Natl Inst Viral Dis Control & Prevent, State Key Lab Mol Virol & Genet Engn, Beijing 100052, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 366卷 / 03期

基金：

中国国家自然科学基金;

关键词：

influenza virus; H5N1; SELEX; DNA aptamer; antiviral activity;

D O I：

10.1016/j.bbrc.2007.11.183

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

New therapeutics are urgently needed for the treatment of pandemic influenza caused by H5N1 influenza virus mutants. Aptamer was a promising candidate for treatment and prophylaxis of influenza virus infections. In this study, systemic evolution of ligands through exponential enrichment (SELEX) was used to screen DNA aptamers targeted to recombinant HA1 proteins of the H5N1 influenza virus. After 11 rounds of selection, DNA aptamers that bind to the HA1 protein were isolated and shown to have different binding capacities. Among them, aptamer 10 had the strongest binding to the HA1 protein, and had an inhibitory effect on H5N1 influenza virus, as shown 9 by the hemagglutinin and MTT assays. These results should aid the development of new drugs for the prevention and control of influenza virus infections. (c) 2007 Elsevier Inc. All rights reserved.

引用

页码：670 / 674

页数：5

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