Tomato BRASSINOSTEROID INSENSITIVE1 is required for systemin-induced root elongation in Solanum pimpinellifolium but is not essential for wound signaling

被引:74
作者
Holton, Nicholas
Cano-Delgado, Ana
Harrison, Kate
Montoya, Teresa
Chory, Joanne
Bishop, Gerard J. [1 ]
机构
[1] Univ London Imperial Coll Sci & Technol, Div Biol, Ashford TN25 5AN, Kent, England
[2] Univ Wales, Inst Biol Sci, Aberystwyth SY23 3DA, Dyfed, Wales
[3] Salk Inst Biol Studies, Howard Hughes Med Inst, Plant Biol Lab, La Jolla, CA 92037 USA
[4] CSIC, Inst Rec & Tecnol Agroalimentaries, CONSORCIO, Barcelona 08034, Spain
基金
英国生物技术与生命科学研究理事会;
关键词
D O I
10.1105/tpc.106.047795
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The tomato Leu-rich repeat receptor kinase BRASSINOSTEROID INSENSITIVE1 (BRI1) has been implicated in both peptide (systemin) and steroid (brassinosteroid [BR]) hormone perception. In an attempt to dissect these signaling pathways, we show that transgenic expression of BRI1 can restore the dwarf phenotype of the tomato curl3 (cu3) mutation. Confirmation that BRI1 is involved in BIR signaling is highlighted by the lack of BR binding to microsomal fractions made from cu3 mutants and the restoration of BR responsiveness following transformation with BRI1. In addition, wound and systemin responses in the cu3 mutants are functional, as assayed by proteinase inhibitor gene induction and rapid alkalinization of culture medium. However, we observed BRI1-dependent root elongation in response to systemin in Solanum pimpinellifolium. In addition, ethylene perception is required for normal systemin responses in roots. These data taken together suggest that cu3 is not defective in systemin-induced wound signaling and that systemin perception can occur via a non-BRI1 mechanism.
引用
收藏
页码:1709 / 1717
页数:9
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