Evaluation of Escherichia coli Cell Response to Antibiotic Treatment by Use of Raman Spectroscopy with Laser Tweezers

被引:47
作者
Moritz, Tobias J. [1 ,2 ]
Polage, Christopher R. [1 ,3 ]
Taylor, Douglas S. [1 ,4 ]
Krol, Denise M. [1 ,2 ,5 ]
Lane, Stephen M. [1 ]
Chan, James W. [1 ,6 ]
机构
[1] Univ Calif Davis, NSF Ctr Biophoton Sci & Technol, Sacramento, CA 95817 USA
[2] Univ Calif Davis, Biophys Grad Grp, Davis, CA 95616 USA
[3] UC Davis Med Ctr, Dept Pathol & Lab Med, Sacramento, CA 95817 USA
[4] UC Davis Med Ctr, Dept Pediat, Sacramento, CA 95817 USA
[5] Univ Calif Davis, Dept Appl Sci, Davis, CA 95616 USA
[6] Lawrence Livermore Natl Lab, Div Phys, Livermore, CA 94550 USA
基金
美国国家科学基金会;
关键词
RAPID IDENTIFICATION; BACTERIAL;
D O I
10.1128/JCM.01565-10
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Laser tweezers Raman spectroscopy was used to detect the cellular response of Escherichia coli cells to penicillin G-streptomycin and cefazolin. Time-dependent intensity changes of several Raman peaks at 729, 1,245, and 1,660 cm(-1) enabled untreated cells and cells treated with the different antibiotic drugs to be distinguished.
引用
收藏
页码:4287 / 4290
页数:4
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