Light-scattering detection of phospholipids resolved by HPLC

被引：37

作者：

Descalzo, AM ^{[1
]}

Insani, EM ^{[1
]}

Pensel, NA ^{[1
]}

机构：

[1] Ctr Invest Agroind, Inst Tecnol Alimentos, Inst Nacl Tecnol Agropecuaria Castelar, Buenos Aires, DF, Argentina

来源：

LIPIDS | 2003年 / 38卷 / 09期

关键词：

D O I：

10.1007/s11745-003-1154-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An improved method for the analysis of phospholipids by normal-phase HPLC is described. Addition of methanol and acetonitrile to a gradient based on 2-propanol/hexane/water promoted a rapid separation of major classes of bovine surfactant phospholipids (PL) by using a conventional silica column. The use of an ELSD permitted an accurate analysis of a mixture of PL. Calibration curves were linear within the range of 5-40 mug with detection limits below 1 mug for PE and PC, and CV ranged from 0.6 to 9.6%. PL present in surfactant homogenates were separated by a solid-phase extraction (SPE) procedure before HPLC analysis. This methodology gave a recovery of 95% and combined SPE-HPLC and quantification of biological PL within a 30-min run. The use of ELSD detection of the eluted compounds was precise, linear, and sensitive.

引用

页码：999 / 1003

页数：5

共 35 条

[1] Reversed-phase separations of nitrogenous phospholipids on an octadecanoyl poly(vinyl alcohol) phase [J].