Cystic fibrosis sputum supports growth and cues key aspects of Pseudomonas aeruginosa physiology

被引:324
作者
Palmer, KL
Mashburn, LM
Singh, PK
Whiteley, M [1 ]
机构
[1] Univ Oklahoma, Hlth Sci Ctr, Dept Periodont, Oklahoma City, OK 73104 USA
[2] Univ Oklahoma, Hlth Sci Ctr, Dept Microbiol & Immunol, Oklahoma City, OK 73104 USA
[3] Univ Iowa, Coll Med, Div Pulm & Crit Care Med, Iowa City, IA 52240 USA
关键词
D O I
10.1128/JB.187.15.5267-5277.2005
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The opportunistic human pathogen Pseudomonas aeruginosa causes persistent airway infections in patients with cystic fibrosis (CF). To establish these chronic infections, P. aeruginosa must grow and proliferate within the highly viscous sputum in the lungs of CF patients. In this study, we used Affymetrix GeneChip microarrays to investigate the physiology of P. aeruginosa grown using CF sputum as the sole source of carbon and energy. Our results indicate that CF sputum readily supports high-density P. aeruginosa growth. Furthermore, multiple signals, which reduce swimming motility and prematurely activate the Pseudomonas quinolone signal cell-to-cell signaling cascade in P. aeruginosa, are present in CF sputum. P. aeruginosa factors critical for lysis of the common CF lung inhabitant Staphylococcus aureus were also induced in CF sputum and increased the competitiveness of P. aeruginosa during polymicrobial growth in CF sputum.
引用
收藏
页码:5267 / 5277
页数:11
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