Biotin synthase: Enzyme or reactant?

被引：18

作者：

Jarrett, JT ^{[1
]}

机构：

[1] Univ Penn, Dept Biochem & Biophys, Philadelphia, PA 19104 USA

来源：

CHEMISTRY & BIOLOGY | 2005年 / 12卷 / 04期

关键词：

D O I：

10.1016/j.chembiol.2005.04.003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Biotin synthase catalyzes formation of a thiophane ring through a radical mechanism that is difficult to reconstitute in vitro. Choi-Rhee and Cronan [1] measure a turnover of 20-60 equivalents of biotin in vivo, but also find that turnover renders the protein susceptible to proteolytic destruction.

引用

页码：409 / 410

页数：2

共 13 条

[1] Crystal structure of biotin synthase, an S-adenosylmethionine-dependent radical enzyme [J].

Berkovitch, F ;

Nicolet, Y ;

Wan, JT ;

Jarrett, JT ;

Drennan, CL .

SCIENCE, 2004, 303 (5654) :76-79

[2] Further investigation on the turnover of Escherichia coli biotin synthase with dethiobiotin and 9-mercaptodethiobiotin as substrates [J].

Bui, BTS ;

Lotierzo, M ;

Escalettes, F ;

Florentin, D ;

Marquet, A .

BIOCHEMISTRY, 2004, 43 (51) :16432-16441

[3] Biotin synthase is catalytic in vivo, but catalysis engenders destruction of the protein [J].

Choi-Rhee, E ;

Cronan, JE .

CHEMISTRY & BIOLOGY, 2005, 12 (04) :461-468

[4] The [4Fe-4S]2+ cluster in reconstituted biotin synthase binds S-adenosyl-L-methionine [J].

Cosper, MM ;

Jameson, GNL ;

Davydov, R ;

Eidsness, MK ;

Hoffman, BM ;

Huynh, BH ;

Johnson, MK .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (47) :14006-14007

[5] Characterization of the cofactor composition of Escherichia coli biotin synthase [J].

Cosper, MM ;

Jameson, GNL ;

Hernández, HL ;

Krebs, C ;

Huynh, BH ;

Johnson, MK .

BIOCHEMISTRY, 2004, 43 (07) :2007-2021

[6] Biotin synthase mechanism: Evidence for hydrogen transfer from the substrate into deoxyadenosine [J].

Escalettes, F ;

Florentin, D ;

Bui, BTS ;

Lesage, D ;

Marquet, A .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1999, 121 (15) :3571-3578

[7] Formation of iron-sulfur clusters in bacteria: an emerging field in bioinorganic chemistry [J].

Frazzon, J ;

Dean, DR .

CURRENT OPINION IN CHEMICAL BIOLOGY, 2003, 7 (02) :166-173

[8] Role of the [2Fe-2S] cluster in recombinant Escherichia coli biotin synthase [J].

Jameson, GNL ;

Cosper, MM ;

Hernández, HL ;

Johnson, MK ;

Huynh, BH .

BIOCHEMISTRY, 2004, 43 (07) :2022-2031

[9] The novel structure and chemistry of iron-sulfur clusters in the adenosylmethionine-dependent radical enzyme biotin synthase [J].

Jarrett, JT .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2005, 433 (01) :312-321

[10] The generation of 5′-deoxyadenosyl radicals by adenosylmethionine-dependent radical enzymes [J].

Jarrett, JT .

CURRENT OPINION IN CHEMICAL BIOLOGY, 2003, 7 (02) :174-182

← 1 2 →