Advanced laboratory techniques for diagnosing Toxoplasma gondii encephalitis in AIDS patients:: significance of intrathecal production and comparison with PCR and ECL-western blotting

被引：27

作者：

Contini, C

Fainardi, E

Cultrera, R

Canipari, R

Peyron, F

Delia, S

Paolino, E

Granieri, E

机构：

[1] Univ Ferrara, Dept Clin & Expt Med, Infect Dis Sect, I-44100 Ferrara, Italy

[2] Univ Ferrara, Sect Neurol Clin, I-44100 Ferrara, Italy

[3] Univ La Sapienza, Dept Trop & Infect Dis, I-00161 Rome, Italy

[4] Univ La Sapienza, Dept Histol & Embriol, I-00161 Rome, Italy

[5] Univ Lyon 1, Dept Parasitol, F-69373 Lyon, France

来源：

JOURNAL OF NEUROIMMUNOLOGY | 1998年 / 92卷 / 1-2期

关键词：

Toxoplasma gondii encephalitis; AIDS; PCR; intrathecal antibody synthesis; oligoclonal bands; ECL western-blot;

D O I：

10.1016/S0165-5728(98)00160-X

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The polymerase chain reaction (PCR) for detection of cerebral spinal fluid (CSF) Toxoplasma gondii DNA was combined with the study of intrathecal antibody synthesis by antibody specific index calculation (ASI) and the detection of specific oligoclonal IgG bands (OCB) by affinity mediated immunoblotting (AMI) in 11 AIDS patients with T. gondii encephalitis (TE) and in 20 control patients with or without neurological disorders. Enhanced chemiluminescence (ECL) western-blot technique was employed to evaluate the antigenic specificity of CSF-IgG towards individual T. gondii antigens. PCR was positive in all TE patients which displayed brain-derived or blood-derived specific OCB, even when comparative ASI failed. Four TE patients had a unique anti-T. gondii OCB restricted to the CSF and a strong antibody response toward the 29 kDa band by ECL western blot. This response could be an important marker to discriminate TE from other opportunistic central nervous system (CNS) infections in the course of AIDS. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：29 / 37

页数：9

共 38 条

[11] KELLOG DE, 1990, PCR PROTOCOLS GUIDE, V90, P339
[12] OLIGOCLONAL IGG BANDS IN CEREBROSPINAL-FLUID - PRINCIPLES FOR DEMONSTRATION AND INTERPRETATION BASED ON FINDINGS IN 1114 NEUROLOGICAL PATIENTS
KOSTULAS, VK
LINK, H
LEFVERT, AK
[J]. ARCHIVES OF NEUROLOGY, 1987, 44 (10) : 1041 - 1044
[13] AVOIDING FALSE POSITIVES WITH PCR
KWOK, S
HIGUCHI, R
[J]. NATURE, 1989, 339 (6221) : 237 - 238
[14] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4
LAEMMLI, UK
[J]. NATURE, 1970, 227 (5259) : 680 - +
[15] CHRONIC HIV ENCEPHALITIS .1. CEREBROSPINAL-FLUID DIAGNOSIS
LUER, W
POSER, S
WEBER, T
JURGENS, S
EICHENLAUB, D
POHLE, HD
FELGENHAUER, K
[J]. KLINISCHE WOCHENSCHRIFT, 1988, 66 (01): : 21 - 25
[16] TOXOPLASMIC ENCEPHALITIS IN AIDS
LUFT, BJ
REMINGTON, JS
[J]. CLINICAL INFECTIOUS DISEASES, 1992, 15 (02) : 211 - 222
[17] Markwell M A, 1981, Methods Enzymol, V72, P296
[18] A comparison of two methods of gene amplification for the diagnosis of Toxoplasma gondii in AIDS
Nicoll, S
Burns, SM
Brettle, RP
Leen, CSL
[J]. JOURNAL OF INFECTION, 1996, 33 (03) : 177 - 183
[19] POLYMERASE CHAIN-REACTION FOR TOXOPLASMA-GONDII DNA IN THE CEREBROSPINAL-FLUID OF AIDS PATIENTS WITH FOCAL BRAIN-LESIONS
NOVATI, R
CASTAGNA, A
MORSICA, G
VAGO, L
TAMBUSSI, G
GHEZZI, S
GERVASONI, C
BISSON, C
MONFORTE, AD
LAZZARIN, A
[J]. AIDS, 1994, 8 (12) : 1691 - 1694
[20] PELLOUX H, 1992, AIDS, V6, P885, DOI 10.1097/00002030-199208000-00022

← 1 2 3 4 →