A surface-based mass spectrometry method for screening glycosidase specificity in environmental samples

被引：17

作者：

Beloqui, Ana ^{[1
]}

Sanchez-Ruiz, Antonio ^{[1
]}

Martin-Lomas, Manuel ^{[1
,2
]}

Reichardt, Niels-Christian ^{[1
,2
]}

机构：

[1] CICbiomaGUNE, Biofunct Nanomat Dept, San Sebastian 20009, Spain

[2] CIBER BBN, San Sebastian 20009, Spain

来源：

CHEMICAL COMMUNICATIONS | 2012年 / 48卷 / 11期

关键词：

ENZYME-ACTIVITY; HYDROLASES; ARRAY;

D O I：

10.1039/c2cc16537f

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A new surface-based MALDI-Tof-MS glycosyl hydrolase assay has been developed in which lipid-tagged oligosaccharides, representing defined fragments of major plant cell wall polysaccharides, are immobilized via hydrophobic interactions on an alkylthiol functionalised gold sample plate and employed in the functional screening of several purified enzymes, environmental samples and saliva.

引用

页码：1701 / 1703

页数：3

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