The eukaryotic gene transcription machinery

被引：16

作者：

Kornberg, RD ^{[1
]}

机构：

[1] Stanford Univ, Sch Med, Dept Biol Struct, Stanford, CA 94305 USA

来源：

BIOLOGICAL CHEMISTRY | 2001年 / 382卷 / 08期

关键词：

electron microscopy; general transcription factors; mediator; RNA polymerase II; two-dimensional crystals; X-ray crystallography;

D O I：

10.1515/BC.2001.140

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Seven purified proteins may be combined to reconstitute regulated, promoter-dependent RNA polymerase II transcription: five general transcription factors, Mediator, and RNA polymerase II. The entire system has been conserved across species from yeast to humans. The structure of RNA polymerase II, consisting of 10 polypeptides with a mass of about 500 kDa, has been determined at atomic resolution. On the basis of this structure, that of an actively transcribing RNA polymerase II complex has been determined as well.

引用

页码：1103 / 1107

页数：5

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