A helical lid converts a sulfotransferase to a dehydratase

被引：25

作者：

Pakhomova, S

Kobayashi, M

Buck, J

Newcomer, ME

机构：

[1] Vanderbilt Univ, Sch Med, Dept Biochem, Nashville, TN 37232 USA

[2] Cornell Univ, Weill Med Coll, Dept Pharmacol, New York, NY 10021 USA

来源：

NATURE STRUCTURAL BIOLOGY | 2001年 / 8卷 / 05期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

D O I：

10.1038/87617

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report here the crystal structure of retinol dehydratase, an enzyme that catalyzes the synthesis of anhydroretinol. The enzyme is a member of the sulfotransferase superfamily and its crystal structure reveals the insertion of a helical lid into a canonical sulfotransferase fold. Site-directed mutations demonstrate that this inserted lid is necessary for anhydroretinol production but not for sulfonation; thus, insertion of a helical lid can convert a sulfotransferase into a dehydratase.

引用

收藏

页码：447 / 451

页数：5

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