Transcriptome of channel catfish (Ictalurus punctatus):: initial analysis of genes and expression profiles of the head kidney

被引：54

作者：

Cao, D

Kocabas, A

Ju, Z

Karsi, A

Li, P

Patterson, A

Liu, Z ^{[1
]}

机构：

[1] Auburn Univ, Dept Fisheries & Allied Aquacultures, Fish Mol Genet & Biotechnol Lab, Auburn, AL 36849 USA

[2] Auburn Univ, Program Cell & Mol Biosci, Auburn, AL 36849 USA

来源：

ANIMAL GENETICS | 2001年 / 32卷 / 04期

关键词：

cDNA; expressed sequence tag (EST); functional genomics; gene expression; mapping; marker; microarray;

D O I：

10.1046/j.1365-2052.2001.00753.x

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Analysis of expressed sequence tags (ESTs) is an efficient approach for gene discovery, expression profiling, and development of resources useful for functional genomics studies. As part of the transcriptome analysis in channel catfish (Ictalurus punctatus), we have conducted EST analysis using a cDNA library made from the head kidney. We analysed 2228 EST clones. Orthologues were established for 1495 (67.1%) clones representing 748 genes, of which 545 (36.5%) clones were singletons. The remaining 733 (32.9%) clones represent unknown gene clones, for which the number of genes has not yet been determined.

引用

页码：169 / 188

页数：20

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