Hyperbranched and monodisperse macromolecules of nanodimensions, commonly referred to as dendrimers, have offered significant potential in addressing key issues in biology. In addition, their monodisperse nature and a generally described globular architecture with high surface group density, make them very coveted candidates as antimicrobial agents. Here, we provide an overview of what has been accomplished in exploring the potential of dendrimers as bactericides, as well as an analysis of the factors influencing their biocidal activity.