Production of apoferritin-based bioinorganic hybrid nanoparticles by bacterial fermentation followed by self-assembly

Apoferritin based bioinorganic hybrid nanoparticles production was analyzed by fermentation followed by self assembly. Hybrid nanoparticles that combine biological and inorganic materials were used in applications such as imaging, biosensing, and gene and drug delivery. Transmission electron microscopy (TEM) showed that the bioinorganic hybrid nanoparticles were homogeneous. The BCCP- ferttine particles was determined by binding BCCP- ferttin to streptavidin coated microtiter wells. These bioinorganic hybrid nanoparticles are cost efficient and simple to produce, which is important when these nanoparticles are used in application. The result shows that it is possible to produce entire functional bioinorganic hybrid nanoparticles in a one bacterial fermentation followed by simple, non chromatographic downstream processing.