Comparison of sequence analysis and INNO-LiPA HBV DR line probe assay in patients with chronic hepatitis B

被引：19

作者：

Sertoz, RY ^{[1
]}

Erensoy, S

Pas, S

Akarca, US

Ozgenc, F

Yamazhan, T

Ozacar, T

Niesters, HGM

机构：

[1] Ege Univ, Fac Med, Dept Clin Microbiol, Izmir, Turkey

[2] Ege Univ, Fac Med, Dept Gastroenterol, Izmir, Turkey

[3] Ege Univ, Fac Med, Dept Pediat, Izmir, Turkey

[4] Ege Univ, Fac Med, Dept Infect Dis, Izmir, Turkey

[5] Erasmus MC, Rotterdam, Netherlands

来源：

JOURNAL OF CHEMOTHERAPY | 2005年 / 17卷 / 05期

关键词：

HBV; lamivudine resistance; sequencing; line probe assay;

D O I：

10.1179/joc.2005.17.5.514

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The aim of this study was to compare direct sequence analysis of partial HBV pol gene and Inno-LiPA HBV DR in serum samples of 120 chronic hepatitis B patients sent to the Clinical Microbiology Laboratory of Ege University Hospital because of lamivudine resistance. Sequence analysis was performed on ABI Prism 310 Genetic Analyzer. Comparison of Inno-LiPA and sequence results obtained by double-blind evaluation showed full agreement (both at rt180 and rt204) in 58.8% of samples. Visually re-checking of the electropherograms increased this rate to 68.3%. Codon based rates are 81.7% and 75.8% at rt180 and rt204 respectively. LiPA detected variants in additional 12 (10%) samples, but missed one variant sample (both rt180 and rt204) and one sample was indeterminate due to poor probe binding. LiPA allows determination of mixed variants and seems to be more sensitive and simple for routine testing even though sequence analysis is still the gold standard for detecting new variants.

引用

页码：514 / 520

页数：7

共 20 条

[1] Comparison of sequence analysis and the INNO-LiPA HBV DR line probe assay for detection of lamivudine-resistant hepatitis B virus strains in patients under various clinical conditions [J].