Molecular characterization of the mycobacterial SenX3-RegX3 two-component system: evidence for autoregulation

被引：60

作者：

Himpens, S ^{[1
]}

Locht, C ^{[1
]}

Supply, P ^{[1
]}

机构：

[1] Inst Pasteur, INSERM, U449, Inst Biol, F-59019 Lille, France

来源：

MICROBIOLOGY-SGM | 2000年 / 146卷

关键词：

two-component systems; autoregulation; phosphorylation; Mycobacterium tuberculosis;

D O I：

10.1099/00221287-146-12-3091

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Environmental regulation of bacterial gene expression is often mediated by two-component signal transduction systems, which are themselves tightly regulated. The response regulator RegX3 and the cytoplasmic portion of the histidine kinase SenX3 from Mycobacterium bovis BCC were overproduced in Escherichia coli and purified as N-terminally (His)(6)-tagged proteins. Phosphorylation assays demonstrated autophosphorylation of the cytoplasmic portion of SenX3 and a phosphotransfer from SenX3 to RegX3, involving conserved histidine and aspartate residues, respectively. In addition, as shown by electrophoretic mobility shift assays, (His),RegX3 was able to specifically bind to the promoter region of the senX3-regX3 operon. Furthermore, operon fusion analyses indicated that the overexpression of the senX3-regX3 operon increases the activity of the senX3 promoter in Mycobacterium smegmatis. Together, these results indicate that the mycobacterial SenX3-RegX3 two-component system is positively autoregulated.

引用

页码：3091 / 3098

页数：8

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