AMID Mediates Adenosine-Induced Caspase-Independent HuH-7 Cell Apoptosis

被引：21

作者：

Yang, Dongqin ^{[1
,2
]}

Yaguchi, Takahiro ^{[2
]}

Nagata, Tetsu ^{[2
]}

Gotoh, Akinobu ^{[3
]}

Dovat, Sinisa ^{[4
]}

Song, Chunhua ^{[4
]}

Nishizaki, Tomoyuki ^{[2
]}

机构：

[1] Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, Shanghai, Peoples R China

[2] Hyogo Coll Med, Dept Physiol, Div Bioinformat, Mukogawa Cho, Hyogo, Japan

[3] Hyogo Coll Med, Inst Adv Med Sci, Lab Cell & Gene Therapy, Mukogawa Cho, Hyogo, Japan

[4] Univ Wisconsin, Sch Med, Dept Pediat, Madison, WI USA

来源：

CELLULAR PHYSIOLOGY AND BIOCHEMISTRY | 2011年 / 27卷 / 01期

关键词：

Adenosine; AMID; Caspase-independent; Apoptosis; HuH-7; cell; HUMAN HEPATOMA-CELLS; CANCER CELLS; RECEPTOR AGONIST; PROTEIN-KINASE; DNA-BINDING; FACTOR AIF; IB-MECA; ACTIVATION; DEATH; EXPRESSION;

D O I：

10.1159/000325203

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Background/Aims: The mechanism underlying extra-cellular adenosine-induced caspase-independent apoptosis in HuH-7 human hepatoma cells is not fully understood. The present study investigated the role for apoptosis-inducing factor (AIF)-homologous mitochondrion-associated inducer of death (AMID) in the pathway. Methods: To see the implication of AMID in adenosine-induced HuH-7 cell apoptosis, real-time reverse transcription-polymerase chain reaction (RT-PCR), immunofluorescent cytochemistry, time-laps GFP monitoring, cell cycle analysis, flow cytometry, Western blotting, cell viability assay, and TUNEL staining were carried out. Results: Adenosine upregulated AMID expression in HuH-7 cells, and translocated AMID from the cytosol into the nucleus. Adenosine induced HuH-7 cell apoptosis, and the effect was further enhanced by overexpressing AMID. Adenosine-induced HuH-7 cell apoptosis, alternatively, was inhibited by knocking-down AMID. Conclusion: The results of the present study provide evidence for AMID as a critical factor for adenosine-induced caspase-independent HuH-7 cell apoptosis. Copyright (C) 2011 S. Karger AG, Basel

引用

页码：37 / 44

页数：8

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