Adaptive functional divergence among triplicated α-globin genes in rodents

被引:28
作者
Storz, Jay F. [1 ]
Hoffmann, Federico G. [1 ]
Opazo, Juan C. [1 ]
Moriyama, Hideaki [2 ]
机构
[1] Univ Nebraska, Sch Biol Sci, Lincoln, NE 68588 USA
[2] Univ Nebraska, Dept Chem, Lincoln, NE 68588 USA
关键词
D O I
10.1534/genetics.107.080903
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The functional divergence of duplicated genes is thought to play an important role in the evolution of new developmental and physiological pathways, but the rote of positive selection in driving this process remains controversial. The objective of this study was to test whether amino acid differences among triplicated alpha-globin paralogs of the Norway rat (Raltus norvegicus) and the deer mouse (Peromyscus maniculatus) are attributable Loa relaxation of purifying selection or to ahistoiy of positive selection that has adapted the gene products to new or modified physiological tasks. In each rodent species, the two paralogs at the 5'-end of the alpha-globin gene cluster (HBA-T1 and HBA-T2) are evolving in concert and are therefore identical or nearly identical in sequence. However, in each case, the HBA-T1 and HBA-T2 paralogs are distinguished from the third paralog at the 3'-end of the gene cluster (HBA-T3) by multiple amino acid substitutions. An analysis of genomic sequence data from several rodent species revealed that the HBA-T3 genes of Rattus and Peromyscus originated via independent, lineage-specific duplication events. In the independently derived HBA-T3 genes of both species, a likelihood analysis based on a codon-substitution model revealed that accelerated rates of amino acid substitution are attributable to positive directional selection, not to a relaxation of purifying selection. As a result of functional divergence among the triplicated alpha-globin genes in Rattus and Peromyscus, the red blood cells of both rodent species contain a mixture of functionally distinct alpha-chain hemoglobin isoforms that are predicted to have different oxygen-binding affinities. In P. maniculatus, a species that is able to sustain physiological function tinder conditions of chronic hypoxia at high altitude, the coexpression of distinct hemoglobin isoforms with graded oxygen affinities is expected to broaden the permissible range of arterial oxygen tensions for pulmonary/tissue oxygen transport.
引用
收藏
页码:1623 / 1638
页数:16
相关论文
共 115 条
[1]   Gene conversion and functional divergence in the β-globin gene family [J].
Aguileta, G ;
Bielawski, JP ;
Yang, ZH .
JOURNAL OF MOLECULAR EVOLUTION, 2004, 59 (02) :177-189
[2]   Proposed standard nomenclature for the α- and β-globin gene families [J].
Aguileta, Gabriela ;
Bielawski, Joseph P. ;
Yang, Ziheng .
GENES & GENETIC SYSTEMS, 2006, 81 (05) :367-371
[3]   Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].
Altschul, SF ;
Madden, TL ;
Schaffer, AA ;
Zhang, JH ;
Zhang, Z ;
Miller, W ;
Lipman, DJ .
NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402
[4]  
Anisimova M, 2003, GENETICS, V164, P1229
[5]   THE CCP4 SUITE - PROGRAMS FOR PROTEIN CRYSTALLOGRAPHY [J].
BAILEY, S .
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1994, 50 :760-763
[6]   The Protein Data Bank [J].
Berman, HM ;
Westbrook, J ;
Feng, Z ;
Gilliland, G ;
Bhat, TN ;
Weissig, H ;
Shindyalov, IN ;
Bourne, PE .
NUCLEIC ACIDS RESEARCH, 2000, 28 (01) :235-242
[7]  
Bielawski J.P., 2003, Genome Evolution, P201, DOI DOI 10.1007/978-94-010-0263-9_20
[8]   A maximum likelihood method for detecting functional divergence at individual codon sites, with application to gene family evolution [J].
Bielawski, JP ;
Yang, ZH .
JOURNAL OF MOLECULAR EVOLUTION, 2004, 59 (01) :121-132
[9]  
CHAPPELL MA, 1988, EVOLUTION, V42, P681, DOI 10.1111/j.1558-5646.1988.tb02486.x
[10]   BIOCHEMICAL AND PHYSIOLOGICAL CORRELATES OF DEER MOUSE ALPHA-CHAIN HEMOGLOBIN POLYMORPHISMS [J].
CHAPPELL, MA ;
SNYDER, LRG .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (17) :5484-5488