Solid-phase synthesis of the exposed capsid peptide VP2(96-107) of the hepatitis a virus. Study of its physicochemical interactions with phospholipids

被引：15

作者：

Haro, I ^{[1
]}

Perez, JA ^{[1
]}

Reig, F ^{[1
]}

Mestres, C ^{[1
]}

Egea, MA ^{[1
]}

Alsina, MA ^{[1
]}

机构：

[1] FAC PHARM, PHYSICOCHEM UNIT, BARCELONA 08028, SPAIN

来源：

LANGMUIR | 1996年 / 12卷 / 21期

关键词：

D O I：

10.1021/la951016j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The synthesis on a solid support of a dodecapeptide containing a HAV-VP2 exposed sequence is described. The surface activity of this peptide as well as its miscibility and interactions with dipalmitoylphosphatidylcholine (DPPC), dipalmitoylphosphatidylethanolamine (DPPE), and phosphatidylinositol (PI) were determined. Interaction energies calculated applying the Gibbs equation gave very small values, thus suggesting either ideal miscibility or weak interactions. In all cases the electrical charge in DPPE and PI monolayers seems to create a high electrostatic potential, decreasing its interaction with any external molecule. This peptide was able to accommodate a certain amount of anilinonaphthalenesulfonate molecules, showing an intermediate hydrophobic character. Moreover, after incubation with saturated liposomes, the peptide showed a clear rigidifying effect at the level of polar heads, especially at temperatures under the transition temperature.

引用

页码：5120 / 5125

页数：6

共 16 条

[1] INTERACTION ENERGIES OF CHOLESTEROL, PHOSPHATIDYLSERINE, AND PHOSPHATIDYLCHOLINE IN SPREAD MIXED MONOLAYERS AT THE AIR-WATER-INTERFACE [J].