Multidimensional proteomics for cell biology

被引：309

作者：

Larance, Mark ^{[1
]}

Lomond, Angus I. ^{[1
]}

机构：

[1] Univ Dundee, Coll Life Sci, Ctr Gene Regulat & Express, Lab Quantitat Prote, Dundee DD1 5EH, Scotland

来源：

NATURE REVIEWS MOLECULAR CELL BIOLOGY | 2015年 / 16卷 / 05期

基金：

英国惠康基金;

关键词：

HYDROPHILIC-INTERACTION CHROMATOGRAPHY; SPECTROMETRY-BASED PROTEOMICS; MASS-SPECTROMETRY; PROTEIN COMPLEXES; QUANTITATIVE PROTEOMICS; SACCHAROMYCES-CEREVISIAE; SYSTEMATIC ANALYSIS; CROSS-LINKING; IDENTIFICATION; PHOSPHORYLATION;

D O I：

10.1038/nrm3970

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

The proteome is a dynamic system in which each protein has interconnected properties-dimensions-that together contribute to the phenotype of a cell. Measuring these properties has proved challenging owing to their diversity and dynamic nature. Advances in mass spectrometry-based proteomics now enable the measurement of multiple properties for thousands of proteins, including their abundance, isoform expression, turnover rate, subcellular localization, post-translational modifications and interactions. Complementing these experimental developments are new data analysis, integration and visualization tools as well as data-sharing resources. Together, these advances in the multidimensional analysis of the proteome are transforming our understanding of various cellular and physiological processes.

引用

页码：269 / 280

页数：12

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