Human urinary trypsin inhibitor inhibits the activation of pro-matrix metalloproteinases and proteoglycans release in rabbit articular cartilage

被引：9

作者：

Hashimoto, K

Nagao, Y

Kato, K

Mori, Y

Ito, A

机构：

[1] Mochida Pharmaceut Co Ltd, Biosci Res Lab, Kita Ku, Tokyo 115, Japan

[2] Tokyo Coll Pharm, Dept Biochem, Hachioji, Tokyo 19203, Japan

来源：

LIFE SCIENCES | 1998年 / 63卷 / 03期

关键词：

urinary trypsin inhibitor; matrix metalloproteinases; plasmin; proteoglycans; rabbit articular chondrocytes;

D O I：

10.1016/S0024-3205(98)00261-6

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Treatment of primary cultured chondrocytes from rabbit articular cartilage with interleukin-1 (IL-1)alpha and plasminogen induced the production of pro-matrix metalloproteinase 1 (proMMP-1/interstitial collagenase), proMMP-3 (stromelysin 1) and proMMP-9 (gelatinase B), as well as their active forms. Human urinary trypsin inhibitor (UTI), a multipotent inhibitor of serine proteases, including plasmin inhibited the activation of proMMP-1, proMMP-3 and proMMP-9 when added to the culture medium together with IL-1 alpha and plasminogen, in a dose-dependent manner. Moreover, UTI inhibited the release of proteoglycans induced by IL-1 alpha and plasminogen from rabbit articular cartilage explants. These findings strongly suggest that UTI inhibits the destruction of articular cartilage induced by plasmin and/or MMPs. Thus, UTI probably exert an anti-osteoarthritic action via inactivation of proMMPs.

引用

页码：205 / 213

页数：9