A new and versatile method for the successful conversion of AFLP™ markers into simple single locus markers -: art. no. e55

被引：86

作者：

Brugmans, B ^{[1
]}

van der Hulst, RGM ^{[1
]}

Visser, RGF ^{[1
]}

Lindhout, P ^{[1
]}

van Eck, HJ ^{[1
]}

机构：

[1] Univ Wageningen & Res Ctr, Dept Plant Sci, Lab Plant Breeding, Grad Sch Expt Plant Sci, NL-6700 AJ Wageningen, Netherlands

来源：

NUCLEIC ACIDS RESEARCH | 2003年 / 31卷 / 10期

关键词：

D O I：

10.1093/nar/gng055

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Genetic markers can efficiently be obtained by using amplified fragment length polymorphism (AFLP) fingerprinting because no prior information on DNA sequence is required. However, the conversion of AFLP markers from complex fingerprints into simple single locus assays is perceived as problematic because DNA sequence information is required for the design of new locus-specific PCR primers. In addition, single locus polymorphism (SNP) information is required to design an allele-specific assay. This paper describes a new and versatile method for the conversion of AFLP markers into simple assays. The protocol presented in this paper offers solutions for frequently occurring pitfalls and describes a procedure for the identification of the SNP responsible for the AFLP. By following this approach, a high success rate for the conversion of AFLP markers into locus-specific markers was obtained.

引用

页数：9

共 33 条

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