Tyrosine phosphorylation in Myxococcus xanthus, a multicellular prokaryote

Tyrosine phosphorylation is an extremely rare event in prokaryotes, occurring almost exclusively in multi-cellular eukaryotes. We have identified, for the first time, by the use of antiphosphotyrosine monoclonal antibody and Western biot (immunoblot) analysis, two tyrosine-phosphorylated membrane proteins in the multicellular prokaryote Myxococcus xanthus. The pattern of tyrosine phosphorylation was shown to change during development, indicating a possible role for this regulatory modification during two stages of development, i.e., aggregation and sporulation. Furthermore, the altered pattern of tyrosine phosphorylation observed ina variety of signaling mutants was shown to differ from that observed in the wild type, suggesting further the possible involvement of tyrosine phosphorylation during the development program.