Regulation and function of Dbx genes in the zebrafish spinal cord

被引：37

作者：

Gribble, Suzanna L. ^{[1
,2
]}

Nikolaus, O. Brant ^{[1
]}

Dorsky, Richard I. ^{[1
,2
]}

机构：

[1] Univ Utah, Sch Med, Dept Neurobiol & Anat, Salt Lake City, UT 84132 USA

[2] Univ Utah, Neurosci Program, Salt Lake City, UT 84112 USA

来源：

DEVELOPMENTAL DYNAMICS | 2007年 / 236卷 / 12期

关键词：

Dbx; spinal cord; zebrafish; Hedgehog; retinoic acid;

D O I：

10.1002/dvdy.21367

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

Dbx homeodomain proteins are important for spinal cord dorsal/ventral patterning and the production of multiple spinal cord cell types. We have examined the regulation and function of Dbx genes in the zebrafish. We report that Hedgehog signaling is not required for the induction or maintenance of these genes; in the absence of Hedgehog signaling, dbx1a/1b/2 are expanded ventrally with concomitant increases in postmitotic neurons that differentiate from this domain. Also, we find that retinoic acid signaling is not required for the induction of Dbx expression. Furthermore, we are the first to report that knockdown of Dbx1 function causes a dorsal expansion of nkx6.2, which is thought to be the cross-repressive partner of Dbx1 in mouse. Our data confirm that the dbx1a/1b/2 domain in zebrafish spinal cord development behaves similarly to amniotes, while extending knowledge of Dbx1 function in spinal cord patterning.

引用

页码：3472 / 3483

页数：12

共 49 条

[41] The zebrafish Pax3 and Pax7 homologues are highly conserved, encode multiple isoforms and show dynamic segment-like expression in the developing brain [J].

Seo, HC ;

Sætre, BO ;

Håvik, B ;

Ellingsen, S ;

Fjose, A .

MECHANISMS OF DEVELOPMENT, 1998, 70 (1-2) :49-63

[42] Three structurally and functionally conserved Hlx genes in zebrafish [J].

Seo, HC ;

Nilsen, F ;

Fjose, A .

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 1999, 1489 (2-3) :323-335

[43] Retinoid-X receptor signalling in the developing spinal cord [J].

Solomin, L ;

Johansson, CB ;

Zetterström, RH ;

Bissonnette, RP ;

Heyman, RA ;

Olson, L ;

Lendahl, U ;

Frisén, J ;

Perlmann, T .

NATURE, 1998, 395 (6700) :398-402

[44] Complementary domains of retinoic acid production and degradation in the early chick embryo [J].

Swindell, EC ;

Thaller, C ;

Sockanathan, S ;

Petkovich, M ;

Jessell, TM ;

Eichele, G .

DEVELOPMENTAL BIOLOGY, 1999, 216 (01) :282-296

[45] Zebrafish evx1 is dynamically expressed during embryogenesis in subsets of interneurones, posterior gut and urogenital system [J].

Thaëron, C ;

Avaron, F ;

Casane, D ;

Borday, V ;

Thisse, B ;

Thisse, C ;

Boulekbache, H ;

Laurenti, P .

MECHANISMS OF DEVELOPMENT, 2000, 99 (1-2) :167-172

[46]

Timmer JR, 2002, DEVELOPMENT, V129, P2459

[47] Different levels of repressor activity assign redundant and specific roles to Nkx6 genes in motor neuron and interneuron specification [J].

Vallstedt, A ;

Muhr, J ;

Pattyn, A ;

Pierani, A ;

Mendelsohn, M ;

Sander, M ;

Jessell, TM ;

Ericson, J .

NEURON, 2001, 31 (05) :743-755

[48] A direct requirement for Hedgehog signaling for normal specification of all ventral progenitor domains in the presumptive mammalian spinal cord [J].

Wijgerde, M ;

McMahon, JA ;

Rule, M ;

McMahon, AP .

GENES & DEVELOPMENT, 2002, 16 (22) :2849-2864

[49] Bmp and Wnt/β-catenin signals control expression of the transcription factor Olig3 and the specification of spinal cord neurons [J].

Zechner, Dietmar ;

Mueller, Thomas ;

Wende, Hagen ;

Walther, Ingrid ;

Taketo, Makoto M. ;

Crenshaw, E. Bryan, III ;

Treier, Mathias ;

Birchmeier, Walter ;

Birchmeier, Carmen .

DEVELOPMENTAL BIOLOGY, 2007, 303 (01) :181-190

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