Overexpression of a plant reticulon remodels the lumen of the cortical endoplasmic reticulum but does not perturb protein transport

被引：111

作者：

Tolley, Nicholas ^{[1
]}

Sparkes, Imogen A. ^{[2
]}

Hunter, Paul R. ^{[1
]}

Craddock, Christian P. ^{[1
]}

Nuttall, James ^{[1
]}

Roberts, Lynne M. ^{[1
]}

Hawes, Chris ^{[2
]}

Pedrazzini, Emanuela ^{[3
]}

Frigerio, Lorenzo ^{[1
]}

机构：

[1] Univ Warwick, Dept Biol Sci, Coventry CV4 7AL, W Midlands, England

[2] Oxford Brookes Univ, Sch Life Sci, Oxford OX3 0BP, England

[3] Ist Biol & Biotecnol Agraria Consiglio Nazl Ric, I-20133 Milan, Italy

来源：

TRAFFIC | 2008年 / 9卷 / 01期

基金：

英国生物技术与生命科学研究理事会;

关键词：

Arabidopsis thaliana; endoplasmic reticulum; FRAP; Golgi; organelle shape; plant; protein transport; reticulon; secretory pathway;

D O I：

10.1111/j.1600-0854.2007.00670.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We have cloned a member of the reticulon (RTN) family of Arabidopsis thaliana (RTNLB13). When fused to yellow fluorescent protein (YFP) and expressed in tobacco leaf epidermal cells, RTNLB13 is localized in the endoplasmic reticulum (ER). Coexpression of a soluble ER luminal marker reveals that YFP-tagged, myc-tagged or untagged RTNLB13 induces severe morphological changes to the lumen of the ER. We show, using fluorescence recovery after photobleaching (FRAP) analysis, that RTNLB13 overexpression greatly reduces diffusion of soluble proteins within the ER lumen, possibly by introducing constrictions into the membrane. In spite of this severe phenotype, Golgi shape, number and dynamics appear unperturbed and secretion of a reporter protein remains unaffected.

引用

页码：94 / 102

页数：9

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