Participation of Valine 171 in α-Helix 5 of Bacillus thuringiensis Cry1Ab δ-Endotoxin in Translocation of Toxin into Lymantria dispar Midgut Membranes

被引:10
作者
Alzate, Oscar [1 ,2 ]
Osorio, Cristina [1 ]
Florez, Alvaro M. [3 ]
Dean, Donald H. [1 ]
机构
[1] Ohio State Univ, Dept Biochem, Columbus, OH 43210 USA
[2] Univ Pontificia Bolivariana, Escuela Med, Medellin, Colombia
[3] Univ Santander, Fac Med, Lab Biol Mol & Biotecnol, Bucaramanga, Colombia
关键词
BRUSH-BORDER MEMBRANE; IRREVERSIBLE BINDING; CHANNEL FORMATION; DOMAIN-II; INSERTION; LOOP;
D O I
10.1128/AEM.01428-10
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The Cry1Ab delta-endotoxin V171C mutant protein exhibits a 25-fold increase in toxicity against Lymantria dispar, which correlates with a faster rate of partitioning into the midgut membrane and slightly decreased protein stability. This is an insect-specific mechanism; similar results were not observed in Manduca sexta, another Cry1Ab delta-endotoxin-susceptible insect.
引用
收藏
页码:7878 / 7880
页数:3
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