Participation of Valine 171 in α-Helix 5 of Bacillus thuringiensis Cry1Ab δ-Endotoxin in Translocation of Toxin into Lymantria dispar Midgut Membranes
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作者:
Alzate, Oscar
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Ohio State Univ, Dept Biochem, Columbus, OH 43210 USA
Univ Pontificia Bolivariana, Escuela Med, Medellin, ColombiaOhio State Univ, Dept Biochem, Columbus, OH 43210 USA
Alzate, Oscar
[1
,2
]
Osorio, Cristina
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Ohio State Univ, Dept Biochem, Columbus, OH 43210 USAOhio State Univ, Dept Biochem, Columbus, OH 43210 USA
Osorio, Cristina
[1
]
Florez, Alvaro M.
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Univ Santander, Fac Med, Lab Biol Mol & Biotecnol, Bucaramanga, ColombiaOhio State Univ, Dept Biochem, Columbus, OH 43210 USA
Florez, Alvaro M.
[3
]
Dean, Donald H.
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Ohio State Univ, Dept Biochem, Columbus, OH 43210 USAOhio State Univ, Dept Biochem, Columbus, OH 43210 USA
Dean, Donald H.
[1
]
机构:
[1] Ohio State Univ, Dept Biochem, Columbus, OH 43210 USA
[2] Univ Pontificia Bolivariana, Escuela Med, Medellin, Colombia
The Cry1Ab delta-endotoxin V171C mutant protein exhibits a 25-fold increase in toxicity against Lymantria dispar, which correlates with a faster rate of partitioning into the midgut membrane and slightly decreased protein stability. This is an insect-specific mechanism; similar results were not observed in Manduca sexta, another Cry1Ab delta-endotoxin-susceptible insect.