Human skeletal muscle pyruvate dehydrogenase kinase activity increases after a low-carbohydrate diet

被引:56
作者
Peters, SJ [1 ]
St Amand, TA
Howlett, RA
Heigenhauser, GJF
Spriet, LL
机构
[1] Univ Guelph, Dept Human Biol & Nutr Sci, Guelph, ON N1G 2W1, Canada
[2] McMaster Univ, Dept Med, Hamilton, ON L8N 3Z5, Canada
来源
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM | 1998年 / 275卷 / 06期
关键词
carbohydrate metabolism; beta-hydroxyacyl coenzyme A dehydrogenase; carnitine palmitoyltransferase I; citrate synthase; mitochondrial ATP production;
D O I
10.1152/ajpendo.1998.275.6.E980
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
To characterize human skeletal muscle enzymatic adaptation to a low-carbohydrate, high-fat, and high-protein diet (LCD), subjects consumed a eucaloric diet consisting of 5% of the total energy intake from carbohydrate, 63% from fat, and 33% from protein for 6 days compared with their normal diet (52% carbohydrate, 33% fat, and 14% protein). Biopsies were taken from the vastus lateralis before and after 3 and 6 days on a LCD. Intact mitochondria were extracted from fresh muscle and analyzed for pyruvate dehydrogenase (PDH) kinase, total PDH, and carnitine palmitoyltransferase I activities and mitochondrial ATP production rate (using carbohydrate and fat substrates). beta-Hydroxyacyl CoA dehydrogenase, active PDH (PDH,), and citrate synthase activities were also measured on whole muscle homogenates. PDH kinase (PDHK) was calculated as the absolute value of the apparent first-order rate constant of the inactivation of PDH in the presence of 0.3 mM Mg2+-ATP. PDHK increased dramatically from 0.10 +/- 0.02 min(-1) to 0.35 +/- 0.09 min(-1) at 3 days and 0.49 +/- 0.06 min(-1) after 6 days. Resting PDHa activity decreased from 0.63 +/- 0.17 to 0.17 +/- 0.04 mmol.min(-1).kg(-1) after 6 days on the diet, whereas total PDH activity did not change. Activities for all other enzymes were unaltered by the LCD. In summary, severe deficiency of dietary carbohydrate combined with a twofold increase in dietary fat and protein caused a rapid three- to fivefold increase in PDHK activity in human skeletal muscle. The increased PDHK activity downregulated the amount of PDH in its active form at rest and decreased carbohydrate metabolism. However, an increase in the activities of enzymes involved in fatty acid oxidation did not occur.
引用
收藏
页码:E980 / E986
页数:7
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