A multidisciplinary approach for molecular diagnostics based on biosensors and microarrays

被引:17
作者
Cloarec, J. -P. [1 ]
Chevolot, Y. [1 ]
Laurenceau, E. [1 ]
Phaner-Goutorbe, M. [1 ]
Souteyrand, E. [1 ]
机构
[1] Ecole Cent Lyon, INL, CNRS, UMR 5270, F-69134 Ecully, France
关键词
biosensors; biochips; surface chemistry; molecular diagnostics; microfluidics; DNA; oligonucleotides; peptides; carbohydrates; fluorescence;
D O I
10.1016/j.rbmret.2007.12.008
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
The present review describes the multidisciplinary approach followed by the Chemistry and Nanobiotechnology group of INL since ten years, to develop a complete technological platform dedicated to molecular diagnosis using biochips and biosensors. This work, replaced in an international context, illustrates the importance to identify the various pitfalls inherent to molecular analysis throughout an elaboration and analysis line: choice of solid support, surface physicochemistry, immobilisation of biomolecules, biomolecular recognition, microfluidics and detection. (C) 2008 Elsevier Masson SAS. All rights reserved.
引用
收藏
页码:105 / 127
页数:23
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