High-density oligonucleotide arrays

被引：210

作者：

Blanchard, AP

Kaiser, RJ

Hood, LE

机构：

来源：

BIOSENSORS & BIOELECTRONICS | 1996年 / 11卷 / 6-7期

关键词：

oligonucleotide arrays; DNA hybridization; ink-jet; surface tension;

D O I：

10.1016/0956-5663(96)83302-1

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

We are attempting to produce high-density arrays of surface-bound oligonucleotides for hybridization experiments. Our approach is to mechanically miniaturize conventional DNA synthesis chemistry. Ink-jet printer heads are used to deliver small drops of reagents to a chemically modified silicon dioxide surface, where they react to synthesize DNA. The drops are confined to small areas by a pattern of hydrophobic surface modifications, thus allowing the formation of high-density arrays without mixing between adjacent drops.

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收藏

页码：687 / 690

页数：4

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