Optimising enzyme function by directed evolution

被引：76

作者：

Dalby, PA ^{[1
]}

机构：

[1] UCL, Adv Ctr Biochem Engn, Dept Biochem Engn, London WC1E 7JE, England

来源：

CURRENT OPINION IN STRUCTURAL BIOLOGY | 2003年 / 13卷 / 04期

关键词：

D O I：

10.1016/S0959-440X(03)00101-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The past decade has seen a revolution in our ability to engineer designer enzymes using genetic tools that mimic evolution on a laboratory timescale. Many excellent examples of directed evolution applied to a wide range of enzymes have clearly demonstrated its future role in adapting enzymes for use in the chemical industry. Recent advances in 'smart' library design and computational screening are now permitting much deeper searches of sequence space, which potentially increases the extent to which enzyme function can be modified.

引用

页码：500 / 505

页数：6

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